冰菜铝激活苹果酸转运蛋白基因家族的全基因组鉴定与表达分析

doi:10.14088/j.cnki.issn0439-8114.2026.09.033

湖北农业科学 ›› 2026, Vol. 65 ›› Issue (9): 209-215.doi: 10.14088/j.cnki.issn0439-8114.2026.09.033

冰菜铝激活苹果酸转运蛋白基因家族的全基因组鉴定与表达分析

刘晓寒^a, 刘晓涵^a, 易睿影^a, 宋思涵^a, 苏佳敏^a, 李楠^a, 宋小明^b

华北理工大学,a.生命科学学院; b.基础医学院,河北唐山 063210

收稿日期:2026-03-30 出版日期:2026-09-25 发布日期:2026-09-17
通讯作者: 李楠（1989-）,女,河北晋州人,讲师,主要从事盐生植物耐盐分子遗传机制研究,（电子信箱）392016474@qq.com;共同通信作者,宋小明（1985-）,男,河北承德人,教授,主要从事基因组学与生物信息学研究,（电子信箱）songxm@ncst.edu.cn。
作者简介:刘晓寒（2004-）,女,河北沧州人,在读本科生,专业方向为生物信息,（电子信箱）2446835128@qq.com;共同第一作者,刘晓涵（2005-）,女,河北衡水人,在读本科生,专业方向为生物技术,（电子信箱）nah1202@qq.com
基金资助:
华北理工大学大学生创新创业训练计划项目（S202510081066）

Genome-wide identification and expression analysis of the aluminum-activated malate transporter gene family in ice plant

LIU Xiao-han^a, LIU Xiao-han^a, YI Rui-ying^a, SONG Si-han^a, SU Jia-min^a, LI Nan^a, SONG Xiao-ming^b

a. School of Life Science; b. School of Basic Medical Sciences, North China University of Science and Technology, Tangshan 063210, Hebei, China

Received:2026-03-30 Published:2026-09-25 Online:2026-09-17

摘要/Abstract

摘要： 为阐明铝激活苹果酸转运蛋白（ALMT）基因家族在盐生植物冰菜（Mesembryanthemum crystallinum L.）中的系统进化与功能特征,在全基因组范围内对冰菜ALMT基因家族成员进行系统鉴定,并分析其在盐胁迫下的表达模式。结果表明,冰菜基因组中共鉴定出9个McALMT基因,不均匀分布于5条染色体上。McALMT蛋白等电点差异较大（6.54~9.20）,均不含信号肽,含有5~18个跨膜结构域,主要定位于质膜。系统进化分析表明,McALMT与拟南芥ALMT（AtALMT）蛋白共同聚为5个分支,同一分支内的成员具有相似的基因结构和保守基序。启动子区富含盐胁迫和植物激素响应等顺式作用元件。表达谱分析显示,McALMT2在根、茎、叶中均有表达,且在茎和叶中表达量较高;McALMT1、McALMT8和McALMT9表现出明显的茎组织偏好性。在盐胁迫下,McALMT4在所有时间点均维持较高表达水平,而McALMT1、McALMT2和McALMT7在盐胁迫5 d时表现出明显的时间动态响应模式。

关键词: 冰菜（Mesembryanthemum crystallinum L.）, 铝激活苹果酸转运蛋白（ALMT）, 基因家族, 全基因组, 鉴定, 表达, 生物信息学

Abstract: To elucidate the phylogenetic and functional characteristics of the aluminum-activated malate transporter (ALMT) gene family in the halophyte ice plant (Mesembryanthemum crystallinum L.), a genome-wide systematic identification of ALMT gene family members in ice plant was conducted, and their expression patterns under salt stress were analyzed. The results showed that a total of 9 McALMT genes were identified in the ice plant genome, unevenly distributed on 5 chromosomes. The isoelectric points of McALMT proteins varied greatly (6.54 to 9.20), all lacked signal peptides, contained 5 to 18 transmembrane domains, and were mainly localized to the plasma membrane. Phylogenetic analysis showed that McALMT and Arabidopsis ALMT (AtALMT) proteins were clustered into 5 clades, and members within the same clade had similar gene structures and conserved motifs. The promoter regions were rich in cis-acting elements such as those responding to salt stress and plant hormones. Expression profile analysis showed that McALMT2 was expressed in roots, stems, and leaves, with higher expression levels in stems and leaves; McALMT1, McALMT8, and McALMT9 exhibited obvious stem tissue preference. Under salt stress, McALMT4 maintained a relatively high expression level at all time points, while McALMT1, McALMT2, and McALMT7 exhibited an obvious temporal dynamic response pattern at 5 days of salt stress.

Key words: ice plant (Mesembryanthemum crystallinum L.), aluminum-activated malate transporter (ALMT), gene family, genome-wide, identification, expression, bioinformatics

中图分类号:

Q943.2

刘晓寒, 刘晓涵, 易睿影, 宋思涵, 苏佳敏, 李楠, 宋小明. 冰菜铝激活苹果酸转运蛋白基因家族的全基因组鉴定与表达分析[J]. 湖北农业科学, 2026, 65(9): 209-215.

LIU Xiao-han, LIU Xiao-han, YI Rui-ying, SONG Si-han, SU Jia-min, LI Nan, SONG Xiao-ming. Genome-wide identification and expression analysis of the aluminum-activated malate transporter gene family in ice plant[J]. HUBEI AGRICULTURAL SCIENCES, 2026, 65(9): 209-215.

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冰菜铝激活苹果酸转运蛋白基因家族的全基因组鉴定与表达分析

Genome-wide identification and expression analysis of the aluminum-activated malate transporter gene family in ice plant

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