不同年限丹参根际土壤丛枝菌根真菌多样性

doi:10.14088/j.cnki.issn0439-8114.2025.10.007

摘要/Abstract

摘要： 为探究丹参（Salvia miltiorrhiza Bunge）根际土壤丛枝菌根真菌（AMF）在丹参连作障碍中的作用,采用AMF特异性引物进行两轮PCR扩增并建库,通过高通量测序分析野生、二年生和五年生丹参根际土壤AMF的群落结构及多样性。结果表明,丹参根系具有明显的菌丝、泡囊和孢子结构;二年生及五年生丹参根系的侵染率显著高于野生丹参,孢子密度显著低于野生丹参;共鉴定到7科7属77种AMF。其中,球囊霉属（Glomus）是AMF群落中的绝对优势属,占比为87.94%。冗余分析（RDA）及基于距离的冗余分析（db-RDA）结果表明,二年生丹参根际土壤中的AMF群落α多样性及群落结构与野生丹参和五年生丹参存在明显区别;碱解氮含量和全氮含量分别是影响AMF群落多样性和群落结构的关键因子。相关性分析表明,土壤全氮含量与Shannon多样性指数、ACE指数、OTUs数量均呈显著正相关;碱解氮含量与ACE指数、OTUs数量均呈显著正相关;Glomus相对丰度与Pielou均匀度指数、Shannon多样性指数均呈显著正相关,与有效磷含量呈显著负相关。综上,五年生丹参根际土壤的微生态与野生丹参相似,且氮元素是影响AMF多样性及群落结构的主要土壤因子,有效磷是影响Glomus分布的主要土壤因子。

关键词: 丹参（Salvia miltiorrhiza Bunge）, 栽培年限, 根际土壤, 丛枝菌根真菌（AMF）, 群落多样性, 群落结构, 连作

Abstract: To investigate the role of arbuscular mycorrhizal fungi (AMF) in the continuous cropping obstacles of Salvia miltiorrhiza Bunge, a sequencing library was constructed using a two-round PCR amplification approach with AMF-specific primers, and the community structure and diversity of AMF in the rhizosphere soil of wild, two-year-old, and five-year-old S. miltiorrhiza plants were analyzed by high-throughput sequencing technology. The results showed that the root system of S. miltiorrhiza had obvious mycelium, vesicle and spore structures. The mycorrhizal infestation rate of two-year old and five-year old S. miltiorrhiza root systems was significantly higher than that of wild S. miltiorrhiza, and the spore density was significantly lower than that of wild S. miltiorrhiza. A total of 77 AMF species from 7 families and 7 genera were identified from all the samples, and Glomus was the absolutely dominant genus in the AMF community, accounting for 87.94% of the total. Redundancy analysis (RDA) and distance-based redundancy analysis (db-RDA) revealed significant differences in both α-diversity and community composition of AMF between the rhizosphere soil of two-year-old cultivated S. miltiorrhiza and that of wild or five-year-old cultivated plants. Alkali-hydrolyzable nitrogen and total nitrogen were identified as key factors influencing AMF community diversity and structure, respectively. Correlation analysis revealed that soil total nitrogen content was significantly positively correlated with the Shannon diversity index, the ACE index, and the number of OTUs. Alkali-hydrolyzable nitrogen content showed significant positive correlations with the ACE index and the number of OTUs. The relative abundance of Glomus was significantly positively correlated with the Pielou evenness index and the Shannon diversity index, but significantly negatively correlated with available phosphorus content. In conclusion, the micro-ecological environment of the five-year-old S. miltiorrhiza rhizosphere soil was similar to that of the wild sample. Furthermore, nitrogen was identified as the primary soil factor influencing AMF diversity and community structure, while available phosphorus was the key factor affecting the distribution of Glomus.

Key words: Salvia miltiorrhiza Bunge, cultivation years, rhizosphere soil, arbuscular mycorrhizal fungi (AMF), community diversity, community structure, continuous cropping

中图分类号:

S154.3

李丽, 彭点, 杨浩, 刘瑞博. 不同年限丹参根际土壤丛枝菌根真菌多样性[J]. 湖北农业科学, 2025, 64(10): 41-48.

LI Li, PENG Dian, YANG Hao, LIU Rui-bo. Diversity of arbuscular mycorrhizal fungi in the rhizosphere soil of Salvia miltiorrhiza at different cultivation years[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(10): 41-48.

参考文献

[1] MA X K, ZHANG L, GAO F J, et al.Salvia miltiorrhiza and Tanshinone IIA reduce endothelial inflammation and atherosclerotic plaque formation through inhibiting COX-2[J]. Biomedicine & pharmacotherapy, 2023, 167: 115501.
[2] 王振,李娜,宋晗,等.丹参种植技术研究进展[J].北方园艺,2023(13):126-131.
[3] LI H Q,JIANG X W, MASHIGUCHI K, et al.Biosynthesis and signal transduction of plant growth regulators and their effects on bioactive compound production in Salvia miltiorrhiza(Danshen)[J]. Chinese medicine, 2024, 19(1): 102.
[4] SHI J C, WANG X L, WANG E T.Mycorrhizal symbiosis in plant growth and stress adaptation: From genes to ecosystems[J]. Annual review of plant biology, 2023, 74: 569-607.
[5] TIBBETT M, DAWS M I, RYAN M H. Phosphorus uptake and toxicity are delimited by mycorrhizal symbiosis in P-sensitive Eucalyptus marginata but not in P-tolerant Acacia celastrifolia[J]. Aob plants, 2022, 14(5): plac037.
[6] AHAMMED G J, SHAMSY R, LIU A R, et al.Arbuscular mycorrhizal fungi-induced tolerance to chromium stress in plants[J]. Environmental pollution, 2023, 327: 121597.
[7] BANERJEE S, VAN DER HEIJDEN M G A. Soil microbiomes and one health[J]. Nature reviews microbiology, 2023, 21(1): 6-20.
[8] 何斐,田孝威,雷雨俊,等.连作花魔芋软腐病株与健株根域丛枝菌根真菌群落多样性[J].微生物学报,2022,62(8):3092-3108.
[9] 郭娟娟,杨帆,李佳怡,等.不同磷水平接种AMF与PSB对土壤理化性质、磷酸酶活性、AMF多样性的影响[J].华北农学报,2024,39(6):154-162.
[10] 王幼珊,刘润进.球囊菌门丛枝菌根真菌最新分类系统菌种名录[J].菌物学报,2017,36(7):820-850.
[11] LI T Q, YANG W K, WU S M, et al.Progress and prospects of mycorrhizal fungal diversity in orchids[J]. Frontiers in plant science, 2021, 12: 646325.
[12] LIU H C,JACQUEMYN H, YU S, et al.Mycorrhizal diversity and community composition in co-occurring Cypripedium species[J]. Mycorrhiza, 2023, 33(1): 107-118.
[13] 柳敏,黄文丽,王潇,等.丹参主产区AMF的多样性研究[J].中国中药杂志,2017,42(1):70-75.
[14] WU Y H,WANG H, LIU M, et al.Effects of native arbuscular mycorrhizae isolated on root biomass and secondary metabolites of Salvia miltiorrhiza Bge.[J]. Frontiers in plant science,2021,12: 617892.
[15] 郑玲玲,陈美兰,康利平,等.根内根孢囊霉Rhizophagus intraradices对丹参生长作用的研究[J].中国中药杂志,2023,48(2):349-355.
[16] WU Y H, QIN Y, CAI Q Q, et al.Effect the accumulation of bioactive constituents of a medicinal plant (Salvia miltiorrhiza Bge.) by arbuscular mycorrhizal fungi community[J]. BMC plant biology, 2023, 23(1): 597.
[17] HE C, HAN T T, LIU C, et al.Deciphering the effects of genotype and climatic factors on the performance, active ingredients and rhizosphere soil properties of Salvia miltiorrhiza[J]. Frontiers in plant science, 2023, 14: 1110860.
[18] SONG H, LI N, ZHANG R C, et al.Study on signal transmission mechanism of arbuscular mycorrhizal hyphal network against root rot of Salvia miltiorrhiza[J]. Scientific reports, 2023, 13(1): 16936.
[19] TEDERSOO L, BAHRAM M, ZOBEL M. How mycorrhizal associations drive plant population and community biology[J]. Science, 2020, 367(6480): eaba1223.
[20] MARTIN F M, ÖPIK M, DICKIE I A.Mycorrhizal research now: From the micro-to the macro-scale[J]. The new phytologist, 2024, 242(4): 1399-1403.
[21] FEI S L, KIVLIN S N, DOMKE G M, et al.Coupling of plant and mycorrhizal fungal diversity: Its occurrence, relevance, and possible implications under global change[J]. New phytologist, 2022, 234(6): 1960-1966.
[22] LI W, HU X Y, ZHU C S, et al.Control effect of root exudates from mycorrhizal watermelon seedlings on Fusarium wilt and the bacterial community in continuously cropped soil[J]. Frontiers in plant science, 2023, 14: 1225897.
[23] MA J Q, XIE Y, YANG Y S, et al.AMF colonization affects allelopathic effects of Zea mays L. root exudates and community structure of rhizosphere bacteria[J]. Frontiers in plant science, 2022, 13: 1050104.
[24] ZHANG L, ZHOU J C, GEORGE T S, et al.Arbuscular mycorrhizal fungi conducting the hyphosphere bacterial orchestra[J]. Trends in plant science, 2022, 27(4): 402-411.
[25] ZHANG S, LI S M, MENG L B, et al.Root exudation under maize/soybean intercropping system mediates the arbuscular mycorrhizal fungi diversity and improves the plant growth[J]. Frontiers in plant science, 2024, 15: 1375194.
[26] XIE K, REN Y H, CHEN A Q, et al.Plant nitrogen nutrition: The roles of arbuscular mycorrhizal fungi[J]. Journal of plant physiology, 2022, 269: 153591.
[27] GOVINDARAJULU M, PFEFFER P E, JIN H R, et al.Nitrogen transfer in the arbuscular mycorrhizal symbiosis[J]. Nature, 2005, 435(7043): 819-823.
[28] 陈笑莹,宋凤斌,朱先灿,等.低温胁迫下丛枝菌根真菌对玉米幼苗形态、生长和光合的影响[J].华北农学报,2014,29(S1):155-161.
[29] SHI J C, ZHAO B Y, ZHENG S, et al.A phosphate starvation response-centered network regulates mycorrhizal symbiosis[J]. Cell, 2021, 184(22): 5527-5540.
[30] 刘春雨,杨智宇,李丽丽,等.菌根真菌对植物氮素利用影响研究进展[J].华北农学报,2023,38(S1):348-353.
[31] 国显宝,韩冰,逯新民,等.氮添加对青藏高原高寒草甸丛枝菌根真菌群落的影响[J].草地学报,2024,32(7):2054-2061.
[32] 孙颖盈,王欣雨,祝晨琳,等.光照强度和氮素形态对棉花-丛枝菌根真菌共生体碳氮代谢的影响[J].微生物学杂志,2024, 44(3):32-39.
[33] SMITH S E, READ D.Mycorrhizal symbiosis[M]. 3rd ed. Amsterdam: Elsevier, 2008.