湖北农业科学 ›› 2025, Vol. 64 ›› Issue (10): 99-104.doi: 10.14088/j.cnki.issn0439-8114.2025.10.015

• 植物保护 • 上一篇    下一篇

湖南百合炭疽病病原菌的分离及鉴定

邓桥1, 储昌斌2, 方冬珍1, 蔡道辉2, 高春生1   

  1. 1.中国农业科学院麻类研究所,长沙 410221;
    2.龙山县植保植检站,湖南 龙山 416800
  • 收稿日期:2024-05-29 出版日期:2025-10-25 发布日期:2025-11-14
  • 通讯作者: 高春生(1981-),男,山东淄博人,研究员,主要从事百合病虫害研究,(电子信箱)gaochunsheng@caas.cn。
  • 作者简介:邓 桥(1998-),男,湖南常德人,在读硕士研究生,研究方向为百合真菌病害,(电子信箱)2389526442@qq.com。
  • 基金资助:
    中国农业科学院科技创新工程项目(ASTIP-IBFC-04)

Isolation and identification of pathogen of anthracnose of lily in Hunan Province

DENG Qiao1, CHU Chang-bin2, FANG Dong-zhen1, CAI Dao-hui2, GAO Chun-sheng1   

  1. 1. Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410221, China;
    2. Plant Protection and Inspection Station of Longshan County, Longshan 416800, Hunan, China
  • Received:2024-05-29 Published:2025-10-25 Online:2025-11-14

摘要: 采用传统组织块分离法,对湖南省百合(Lilium brownii var. viridulum Baker)主产区的百合炭疽病病原菌进行分离和纯化,并综合运用形态学和分子生物学方法进行系统鉴定。结果表明,该地区百合炭疽病的主要致病菌为白蜡树炭疽菌(Colletotrichum spaethianum)。致病菌测定证实了分离菌株的致病性,并比较分析了湖南省2种百合品种在病原菌侵染上的差异性。

关键词: 百合(Lilium brownii var. viridulum Baker), 白蜡树炭疽菌(Colletotrichum spaethianum), 分离与鉴定, 聚类分析

Abstract: The pathogen of lily(Lilium brownii var. viridulum Baker) anthracnose in the main production areas of Hunan Province was isolated and purified using the traditional tissue block separation method. Through a combination of morphological and molecular biological methods, it was systematically identified. The results indicated that the primary pathogenic fungus was Colletotrichum spaethianum. The pathogenicity of the isolated strains was confirmed by pathogenicity tests. Additionally, differences in the infection by this pathogen between two cultivated lily varieties in Hunan Province were compared and analyzed.

Key words: lily (Lilium brownii var. viridulum Baker), Colletotrichum spaethianum, isolation and identification, cluster analysis

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