基于相对迁移率的2个山羊草二倍体物种HMW-GS组成分析

doi:10.14088/j.cnki.issn0439-8114.2025.08.027

湖北农业科学 ›› 2025, Vol. 64 ›› Issue (8): 178-182.doi: 10.14088/j.cnki.issn0439-8114.2025.08.027

基于相对迁移率的2个山羊草二倍体物种HMW-GS组成分析

张海惠¹, 张艳茹¹, 宋林轩², 郝志华², 张怡琳¹, 魏飒², 胡喜贵²

1.新乡工程学院生物工程学院,河南新乡 453700;
2.河南科技学院农学院,河南新乡 453003

收稿日期:2025-04-21 出版日期:2025-08-25 发布日期:2025-09-12
通讯作者: 胡喜贵（1981-）,男,河北沧州人,副教授,博士,硕士研究生导师,主要从事作物种质资源挖掘与利用,（电话）18790619695（电子信箱）xiguihu@126.com。
作者简介:张海惠（1987-）,女,内蒙古乌兰察布人,讲师,硕士,主要从事种质资源创新与利用,（电话）15993075909（电子信箱）zhang_hai_hui_@126.com;
基金资助:
河南省科技攻关项目（242102111155; 242102110195）

Analysis of HMW-GS composition of two Aegilops diploid species based on relative mobility

ZHANG Hai-hui¹, ZHANG Yan-ru¹, SONG Lin-xuan², HAO Zhi-hua², ZHANG Yi-lin¹, WEI Sa², HU Xi-gui²

1. School of Biological Engineering, Xinxiang Institute of Engineering, Xinxiang 453700, Henan, China;
2. School of Agriculture, Henan Institute of Science and Technology, Xinxiang 453003, Henan, China

Received:2025-04-21 Published:2025-08-25 Online:2025-09-12

摘要/Abstract

摘要： 山羊草蕴藏着丰富的优异基因或性状,是改良现代普通小麦的重要基因源。为解析山羊草二倍物种高分子量麦谷蛋白亚基（HMW-GS）的遗传多样性,采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）对40份顶芒山羊草（Ae.comosa Sm., MM, 2n=2x=14）和尾状山羊草[Ae. markgraﬁi(Greuter)K. Hammer,CC, 2n=2x=14]的HMW-GS进行系统分析。结果表明,40份材料共鉴定出17种相对迁移率,其中5种迁移率（0.82~0.98）低于中国春1Dx2（1.00）;1种（1.49）高于其1Dy12（1.40）。材料间的遗传相似系数（GS）分析显示,其变化范围为0.00~1.00,平均值为0.23;尾状山羊草材料间GS平均值（0.58）显著高于顶芒山羊草材料间GS（0.31）（P<0.05）。聚类结果显示在GS约0.10处,19份顶芒山羊草材料聚成Group1类,其余21份材料形成Group2类,其中Group2进一步分成2个Sub亚类,表现出种源相同的材料倾向聚在一起。

关键词: 顶芒山羊草（Ae.comosa Sm.,MM,2n=2x=14）, 尾状山羊草[Ae. markgraﬁi(Greuter)K. Hammer,CC,2n=2x=14], HMW-GS, 遗传多样性, 聚类分析

Abstract: Aegilops is considered as an important gene source for improving modern common wheat, which has many excellent genes or traits. In order to understand the genetic diversity of high molecular weight glutenin subunits (HMW-GS) in Ae. diploid species, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was used to analyze HMW-GS in 40 Ae. accession (Ae. comosa and Ae. markgraﬁi). The results showed the total of 17 relative mobilities were identified among the 40 accessions, of which five relative mobilities (0.82~0.98) were lower than that of Chinese Spring 1Dx2 (1.00), and one relative mobility (1.49) was higher than that of Chinese Spring 1Dy12 (1.40). Analysis of genetic similarity coefficients (GS) among the accessions revealed a range of 0.00~1.00, with an average value of 0.23. And, it was found that the average GS (0.58) of Ae. markgraﬁi accessions was significantly higher than that of Ae. comosa accessions(0.31). The clustering results showed that 40 Ae. accessions were divided into two groups at the GS 0.10, of which 19 Ae. comosa accessions were clustered in Group1. And, the Group2 including 21 Ae. accessions could be further divided into two sub-groups, which indicated that Ae. accessions clustering together had the same species.

Key words: Aegilops comosa, Ae. markgraﬁi, HMW-GS, genetic diversity, clustering analysis

中图分类号:

S545

张海惠, 张艳茹, 宋林轩, 郝志华, 张怡琳, 魏飒, 胡喜贵. 基于相对迁移率的2个山羊草二倍体物种HMW-GS组成分析[J]. 湖北农业科学, 2025, 64(8): 178-182.

ZHANG Hai-hui, ZHANG Yan-ru, SONG Lin-xuan, HAO Zhi-hua, ZHANG Yi-lin, WEI Sa, HU Xi-gui. Analysis of HMW-GS composition of two Aegilops diploid species based on relative mobility[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(8): 178-182.

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基于相对迁移率的2个山羊草二倍体物种HMW-GS组成分析

Analysis of HMW-GS composition of two Aegilops diploid species based on relative mobility

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