湖北农业科学 ›› 2025, Vol. 64 ›› Issue (12): 146-151.doi: 10.14088/j.cnki.issn0439-8114.2025.12.024

• 药用植物 • 上一篇    下一篇

赤苍藤茎叶多糖的组成及活性分析

王葳锐1a, 卫炳安1a, 潘乔丹1b,2, 黄元河1a,2,3, 赵丽阳1a, 徐影影1a   

  1. 1.右江民族医学院,a.基础医学院;b.药学院,广西 百色 533000;
    2.广西高校右江流域特色民族药研究重点实验室,广西 百色 533000;
    3.广西金钟山森林生态系统野外科学观测研究站,广西 百色 533000
  • 收稿日期:2025-06-05 发布日期:2025-12-30
  • 作者简介:王葳锐(1997-),女,广东佛山人,在读硕士研究生,研究方向为民族药药效,(电子信箱)240694874@qq.com。
  • 基金资助:
    国家自然科学基金项目(31860094); 广西自然科学基金项目(2025GXNSFHA069101); 百色市科技研究与计划开发课题(百科20183009)

The composition and activity analysis of polysaccharides from Erythropalum scandens Bl. stems and leaves

WANG Wei-rui1a, WEI Bing-an1a, PAN Qiao-dan1b,2, HUANG Yuan-he1a,2,3, ZHAO Li-yang1a, XU Ying-ying1a   

  1. 1. Youjiang Medical University for Nationalities, a. College of Basic Medicine; b. College of Pharmacy, Baise 533000, Guangxi, China;
    2. Key Laboratory of Right River Basin Characteristic Ethnic Medicine Research in Universities of Guangxi, Baise 533000, Guangxi, China;
    3. Guangxi Jinzhongshan Forest Ecosystem Field Scientific Observation and Research Station, Baise 533000, Guangxi, China
  • Received:2025-06-05 Online:2025-12-30

摘要: 采用柱前衍生化高效液相色谱法测定赤苍藤(Erythropalum scandens Bl.)老茎、嫩茎、老叶、嫩叶4种部位多糖的单糖组成,并结合DPPH·、ABTS+·、O2-·(超氧阴离子自由基)的清除能力及铁离子还原能力(FRAP)综合评价其抗氧化性能,以筛选高活性多糖组分。结果表明,赤苍藤多糖主要由10种单糖组成,除嫩茎多糖中未检出木糖外,老茎、老叶和嫩叶多糖均含有甘露糖、氨基葡萄糖、核糖、鼠李糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、半乳糖、木糖和阿拉伯糖;总糖含量高低表现为老茎>嫩茎>嫩叶>老叶。外抗氧化活性试验表明,赤苍藤各部位多糖对DPPH·、ABTS+·、O2-·均具有一定清除能力,并表现出一定的铁离子还原能力,其中老茎多糖的抗氧化活性优于其他部位。

关键词: 赤苍藤(Erythropalum scandens Bl.), 多糖, 单糖分析, 抗氧化活性

Abstract: The monosaccharide composition of polysaccharides from four different parts (old stem, young stem, old leaf, and young leaf) of Erythropalum scandens Bl. was determined using pre-column derivatization high-performance liquid chromatography (HPLC). Their antioxidant properties were comprehensively evaluated by assessing the scavenging capacities against DPPH·, ABTS+·, and superoxide anion radical (O2-·), as well as the ferric ion reducing antioxidant power (FRAP) assay, to screen for highly active polysaccharide components. The results showed that Erythropalum scandens polysaccharides were primarily composed of ten monosaccharides: Namely, mannose, glucosamine, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and arabinose. Xylose was not detected in the young stem polysaccharides, but was present in the old stem, old leaf, and young leaf polysaccharides. The total sugar content followed the order of old stem > young stem > young leaf > old leaf. The in vitro antioxidant assays indicated that polysaccharides from all parts exhibited scavenging activity against DPPH·, ABTS+·, and O2-·, as well as ferric ion reducing power. Among them, the old stem polysaccharide demonstrated superior antioxidant activity compared to the other parts.

Key words: Erythropalum scandens Bl., polysaccharides, monosaccharide analysis, antioxidant activity

中图分类号: