冬凌草总黄酮超高压辅助低共熔溶剂提取及抗氧化特性

doi:10.14088/j.cnki.issn0439-8114.2025.12.031

摘要/Abstract

摘要： 为优化冬凌草(Rabdosia rubescens (Hemsl.) Hara)总黄酮超高压辅助低共熔溶剂（Deep eutectic solvent,DES）提取工艺,并对其抗氧化活性进行分析。通过单因素试验和响应面试验优化提取工艺条件,对提取的黄酮对1,1-二苯基-2-苦肼基（DPPH）和 2,2′-联氨-双（3-乙基苯并噻唑啉-6-磺酸）二铵盐（ABTS）自由基的清除活性进行分析。结果表明,超高压辅助DES提取总黄酮的最佳条件为料液比1∶40（g/mL）、DES含水量30%、提取压力402 MPa和提取时间5 min,该条件下冬凌草总黄酮得率为9.21%。制备得到的冬凌草总黄酮对DPPH·、ABTS·清除作用的半抑制浓度分别为0.136 mg/mL和0.123 mg/mL。研究可为冬凌草总黄酮开发及抗氧化活性研究提供理论支撑。

关键词: 冬凌草(Rabdosia rubescens (Hemsl.) Hara), 总黄酮, 超高压, 低共熔溶剂, 抗氧化

Abstract: To optimize the extraction process of total flavonoids from Rabdosia rubescens (Hemsl.) Hara in super high pressure assisted deep eutectic solvent (DES) and analyze its antioxidant activity, the extraction condition was optimized by single factor experiments and response surface experiments. The scavenging activity of the extracted flavonoids against 1,1-dipyl-2-picrylhydrazyl (DPPH) and 2,2′-azinobis (3-ethylbenzothiazoline-6-ulfonic acid) diammonium salt (ABTS) free radicals was analyzed. The results showed that the optimal condition for the extraction of total flavonoids in super high pressure assisted deep eutectic solvent was material to liquid ratio of 1∶; 40 (g/mL), water content in DES of 30% ,extraction pressure of 402 MPa, and extraction time of 5 min, under which the yield of total flavonoids from the Rabdosia rubescens (Hemsl.) Hara was 9.21%. The half-inhibitory concentrations of the prepared total flavonoids of Rabdosia rubescens (Hemsl.) Hara for the scavenging effects of DPPH· and ABTS·were 0.136 mg/mL and 0.123 mg/mL, respectively. The research could provide theoretical support for the development of total flavonoids of Rabdosia rubescens (Hemsl.) Hara and the study of antioxidant activity.;

Key words: Rabdosia rubescens (Hemsl.) Hara, total flavonoids, super high pressure, deep eutectic solvent, antioxidant

中图分类号:

R284.2

王乐, 张培旗, 纵伟. 冬凌草总黄酮超高压辅助低共熔溶剂提取及抗氧化特性[J]. 湖北农业科学, 2025, 64(12): 181-186.

WANG Le, ZHANG Pei-qi, ZONG Wei. Study on super high pressure assisted deep eutectic solvent extraction of total flavonoids from Rabdosia rubescens (Hemsl.) Hara and its antioxidant activity[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(12): 181-186.

参考文献

[1] 刘海芳,燕树锋,赵霞,等. 冬凌草高效栽培技术[J]. 河南农业, 2025(7): 50-52.
[2] JIAN Z Y,JIAN S S,TANG X M.et al.Photosynthesis capacity and chlorophyll fluorescence characteristics of the Isodon rubescens (Hemsley) H. Hara stem[J]. Scientific reports,2024, 14(11):27598.
[3] 任茹梦,余丽瑞,胡俊杰,等. 冬凌草甲素通过PPARα/CPT-1通路改善非酒精性脂肪肝的作用研究[J]. 天然产物研究与开发, 2025, 37(5): 828-836.
[4] BAI N S, HE K, ZHOU Z, et al.Flavonoids from Rabdosia rubescens exert anti-inflammatory and growth inhibitory effect against human leukemia HL-60 cells[J].Food chemistry,2010,122(3): 831-835.
[5] GUO S, CUI X Q, JIANG M, et al.Simultaneous characterization and quantification of 17 main compounds in Rabdosia rubescens by high performance liquid chromatography[J]. Journal of food and drug analysis,2017, 25(2): 417-424.
[6] GAO S Y, LI J W,WANG W Y,et al.Rabdosia rubescens(Hemsl.) H. Hara: A potent anti-tumor herbal remedy-Botany, phytochemistry, and clinical applications and insights[J]. Journal of ethnopharmacology, 2025, 340(31):119200.
[7] 原勤轩,袁铭潞,由士权,等. 冬凌草发酵液的皮肤抗氧化能力和细胞炎症修复作用[J]. 精细化工, 2025, 42(6): 1323-1330.
[8] 李小霞,刘栋,董靖,等. 冬凌草提取物抑菌杀虫活性和抑菌机制的初步研究[J]. 中国典型病例大全,2025,19(1):408-413.
[9] 范渊,周锐,吴海龙,等. 冬凌草甲素调节cGAS-STING信号通路对重症急性胰腺炎大鼠肠道免疫功能的影响[J]. 中国老年学杂志, 2025, 45(9): 2239-2243.
[10] 梁喜玲,申艳华,刘忭,等. 大麦茶中总黄酮提取方法的对比研究[J]. 农产品加工, 2023(8): 33-34,40.
[11] 王俊龙,蔺永刚,陈凤霞,等. 弯萼金丝桃总黄酮提取及抗氧化、降糖活性[J]. 精细化工, 2024, 41(5): 1050-1059,1083.
[12] 陈胜发,张洁,许海燕. 微波法优选多穗柯中总黄酮的提取工艺研究[J]. 化学研究, 2025, 36(4): 363-370.
[13] WEI P, ZHANG Y, WANG Y Y,et al.Efficient extraction, excellent activity, and microencapsulation of flavonoids from Moringa oleifera leaves extracted by deep eutectic solvent[J]. Biomass conversion and biorefiner,2023,13(1): 15095-15109.
[14] ASHRAF W,REHMAN A,SHARIF H R,et al.Ultrasonication-assisted deep eutectic solvent extraction of flavonoids from pretreated fenugreek and their antidiabetic & hypo-lipidemic potential[J]. Journal of food measurement and characterization,2024,18(5):5397-5410.
[15] LIN Y H, HUANG H W, WANG C Y.Effects of high pressure-assisted extraction on yield, antioxidant, antimicrobial, and anti-diabetic properties of chlorogenic acid and caffeine extracted from green coffee beans[J]. Food and bioprocess technology, 2022,15: 1529-1538.
[16] 王慧, 席小丽. 超高压辅助低共熔溶剂提取复方双黄连有效成分[J].山西大学学报(自然科学版), 2019, 42(3):628-638.
[17] 赵惠茹, 张硕, 王晴, 等. 金银花总黄酮低共熔溶剂提取工艺优化[J].中成药, 2024, 46(5): 1465-1470.
[18] 李娜,武晓英,赵文婧.牛至精油成分分析及其抗氧化性和抑菌活性研究[J].中国调味品,2020,45(9):29-33,54.
[19] 肖咪,罗蓉,赵红玉,等. 超声波辅助低共熔溶剂提取淫羊藿总黄酮的工艺优化及其降糖活性研究[J]. 中国医院药学杂志, 2025,45(2):135-140.
[20] WANG B Q, CHEN P Y, ZHANG H E.et al.Optimization of polyphenols extraction by deep eutectic solvent from broccoli stem and characterization of their composition and antioxidative effects[J].Scientific reports ,2025,15(5): 16066.
[21] ZHAO J M, SU T T, SUN Q Z, et al.Ultrahigh pressure enhances the extraction efficiency, antioxidant potential, and hypoglycemic activity of flavonoids from Chinese sea buckthorn leaves[J].LWT,2024,207(9): 116671.
[22] TANG L Q, LIU H C, WEN J, et al.Study on ultrahigh-pressure extraction technology on properties of yellow extract from gardenia fruit[J].Journal of food composition and analysis,2021,104(12): 104186.
[23] HU W, GUO T, JIANGE W J, et al.Effects of ultrahigh pressure extraction on yield and antioxidant activity of chlorogenic acid and cynaroside extracted from flower buds of Lonicera japonica[J]. Chinese journal of natural medicines,2015,13(6): 445-453.
[24] CHEN Z L, MA J, LI P, et al.Preparation of hypoglycemic anthocyanins from mulberry (Fructus mori) fruits by ultrahigh pressure extraction[J].Innovative food science & emerging technologies, 2023,84(3):103255.
[25] 李传茂,邱志,王枫亮,等.洋甘菊总黄酮提取及其抗氧化活性研究[J].日用化学工业(中英文),2024,54(9): 1039-1049.
[26] CHEN S, WANG X J, CHENG Y, et al.A review of classification, biosynthesis, biological activities and potential applications of flavonoids[J]. Molecules,2023,28(13):4982.