芦竹光合特性与染色体组型分析

doi:10.14088/j.cnki.issn0439-8114.2026.01.029

湖北农业科学 ›› 2026, Vol. 65 ›› Issue (1): 181-185.doi: 10.14088/j.cnki.issn0439-8114.2026.01.029

芦竹光合特性与染色体组型分析

钟楠, 陈虞超, 郭生虎, 刘璇, 田莉, 甘晓燕

宁夏农林科学院农业生物技术研究中心,银川 750021

收稿日期:2024-04-01 出版日期:2026-01-25 发布日期:2026-02-10
通讯作者: 甘晓燕（1982-）,女,宁夏中宁人,硕士,主要从事特色植物资源开发与利用研究,（电子信箱）307937695@qq.com。
作者简介:钟楠（1993-）,女,宁夏银川人,副研究员,主要从事作物遗传育种研究,（电子信箱）zhongnan15@foxmail.com。
基金资助:
宁夏回族自治区重点研发计划项目(2022BBF03033); 宁夏农林科学院农业生物技术研究中心科技创新引导项目

Analysis of photosynthetic characteristics and karyotype in Arundo donax

ZHONG Nan, CHEN Yu-chao, GUO Sheng-hu, LIU Xuan, TIAN Li, GAN Xiao-yan

Agricultural Biotechnology Research Center, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750021, China

Received:2024-04-01 Published:2026-01-25 Online:2026-02-10

摘要/Abstract

摘要： 为明确芦竹(Arundo donax L.)的光合特性与染色体特征,对不同芦竹种质材料的光合模式及染色体组型进行系统鉴定。通过叶片解剖结构观察（扫描电镜）、C₄光合酶活性测定及碳同位素比值（^δ13C）分析综合判定其光合类型,并采用荧光原位杂交（FISH）技术对染色体数量及结构特征进行细胞遗传学鉴定。结果表明,芦竹具有花环结构,表现出典型的C₄植物结构特征。绿洲一号和宁芦1号均在4条染色体上呈现明显的5S rDNA杂交信号,表明两种材料在rDNA位点数量上高度保守。推测绿洲一号、宁芦1号可能均为四倍体或接近九倍体的非整倍体。

关键词: 芦竹(Arundo donax L.), 光合特性, 染色体组型, C₄植物

Abstract: To clarify the photosynthetic characteristics and chromosomal features of Arundo donax, a systematic identification of the photosynthetic pattern and karyotype of different Arundo donax germplasm materials was conducted. The photosynthetic type was comprehensively determined through leaf anatomical structure observation (scanning electron microscopy), C₄ photosynthetic enzyme activity assay, and carbon isotope ratio (^δ13C) analysis, and the chromosome number and structural characteristics were cytogenetically identified using fluorescence in situ hybridization (FISH). The results showed that Arundo donax possessed a Kranz anatomy, exhibiting typical structural characteristics of a C₄ plant. Both Lyuzhou 1 and Ninglu 1 showed clear 5S rDNA hybridization signals on four chromosomes, indicating a high conservation in the number of rDNA loci between the two materials. It was speculated that both Lyuzhou 1 and Ninglu 1 might be tetraploids or aneuploids close to nonaploids.

Key words: Arundo donax, photosynthetic characteristics, karyotype, C₄ plant

中图分类号:

钟楠, 陈虞超, 郭生虎, 刘璇, 田莉, 甘晓燕. 芦竹光合特性与染色体组型分析[J]. 湖北农业科学, 2026, 65(1): 181-185.

ZHONG Nan, CHEN Yu-chao, GUO Sheng-hu, LIU Xuan, TIAN Li, GAN Xiao-yan. Analysis of photosynthetic characteristics and karyotype in Arundo donax[J]. HUBEI AGRICULTURAL SCIENCES, 2026, 65(1): 181-185.

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芦竹光合特性与染色体组型分析

Analysis of photosynthetic characteristics and karyotype in Arundo donax

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