谷子EIN3/EIL基因家族鉴定及其在多组织中的表达分析

doi:10.14088/j.cnki.issn0439-8114.2026.07.033

湖北农业科学 ›› 2026, Vol. 65 ›› Issue (7): 215-220.doi: 10.14088/j.cnki.issn0439-8114.2026.07.033

谷子EIN3/EIL基因家族鉴定及其在多组织中的表达分析

刘鹏程¹, 王亚玉², 杨天天¹

1.东北林业大学野生动物与自然保护地学院,哈尔滨 150040;
2.华大生命科学研究院,广东深圳 518083

收稿日期:2026-03-23 出版日期:2026-07-25 发布日期:2026-07-23
通讯作者: 王亚玉（1983-）,女,湖北武汉人,工程师,主要从事植物微生物互作研究,（电子信箱）wangyayu@genomics.cn。
作者简介:刘鹏程（2001-）,男,吉林长春人,在读硕士研究生,研究方向为植物多组学,（电子信箱）liupengcheng@genomics.cn;杨天天（1979-）,女,黑龙江齐齐哈尔人,工程师,主要从事饲草营养研究,（电子信箱）30441830@qq.com。
基金资助:
国家自然科学基金资助项目（32300265）

Identification of the EIN3/EIL gene family in foxtail millet and its expression analysis in multiple tissues

LIU Peng-cheng¹, WANG Ya-yu², YANG Tian-tian¹

1. College of Wildlife and Protected Area, Northeast Forestry University, Harbin 150040, China;
2. BGI-Research, Shenzhen 518083, Guangdong, China

Received:2026-03-23 Published:2026-07-25 Online:2026-07-23

摘要/Abstract

摘要： 为了系统解析谷子（Setaria italica）EIN3/EIL(SiEIN3/EIL)基因家族的进化特征、结构特点及其在生长发育与环境适应中的潜在调控功能,基于谷子全基因组数据对其进行系统鉴定与多组织表达谱分析。通过HMMER与BLASTP联合检索,共鉴定出4个谷子EIN3/EIL家族成员,基因ID分别为Seita.1G205600.1、Seita.7G121700.1、Seita.2G437500.1、Seita.9G419900.1。生物信息学分析表明,该家族蛋白均定位于细胞核且呈亲水性,分散分布于4条染色体上;所有成员均含有外显子,共享6个核心保守基序,且启动子区域富含光响应、多种植物激素响应（如茉莉酸甲酯、脱落酸等）及非生物胁迫响应等顺式作用元件。多组织转录组数据表达分析显示,各基因的表达水平存在差异,其中,Seita.9G419900.1在拔节期至开花期基因表达量明显高于苗期等营养生长阶段,其主要在生殖发育及花器官形成中发挥核心调控作用。

关键词: 谷子（Setaria italica）, EIN3/EIL基因家族, 鉴定, 组织, 生物信息学, 表达分析

Abstract: In order to systematically elucidate the evolutionary characteristics, structural features, and potential regulatory functions of the EIN3/EIL (SiEIN3/EIL) gene family in foxtail millet (Setaria italica) during growth, development, and environmental adaptation, a systematic identification and multi-tissue expression profile analysis were conducted based on the whole-genome data of foxtail millet. Through combined HMMER and BLASTP searches, a total of four EIN3/EIL family members were identified in foxtail millet, with gene IDs of Seita.1G205600.1, Seita.7G121700.1, Seita.2G437500.1, and Seita.9G419900.1. Bioinformatics analysis indicated that all proteins of this family were localized in the nucleus and were hydrophilic, distributed dispersedly across four chromosomes; all members contained exons, shared six core conserved motifs, and their promoter regions were enriched with cis-acting elements associated with light responses, multiple phytohormone responses (e.g., methyl jasmonate, abscisic acid), and abiotic stress responses. Expression analysis of multi-tissue transcriptome data revealed differences in the expression levels among the genes, among which Seita.9G419900.1 exhibited significantly higher expression from the jointing stage to the flowering stage than during vegetative growth stages such as the seedling stage, playing a core regulatory role mainly in reproductive development and floral organ formation.

Key words: foxtail millet (Setaria italica), EIN3/EIL gene family, identification, tissue, bioinformatics, expression analysis

中图分类号:

S515

刘鹏程, 王亚玉, 杨天天. 谷子EIN3/EIL基因家族鉴定及其在多组织中的表达分析[J]. 湖北农业科学, 2026, 65(7): 215-220.

LIU Peng-cheng, WANG Ya-yu, YANG Tian-tian. Identification of the EIN3/EIL gene family in foxtail millet and its expression analysis in multiple tissues[J]. HUBEI AGRICULTURAL SCIENCES, 2026, 65(7): 215-220.

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谷子EIN3/EIL基因家族鉴定及其在多组织中的表达分析

Identification of the EIN3/EIL gene family in foxtail millet and its expression analysis in multiple tissues

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