硝酸盐对小麦幼苗根系早期生长和抗氧化特性的影响

doi:10.14088/j.cnki.issn0439-8114.2018.23.014

湖北农业科学 ›› 2018, Vol. 57 ›› Issue (23): 61-64.doi: 10.14088/j.cnki.issn0439-8114.2018.23.014

硝酸盐对小麦幼苗根系早期生长和抗氧化特性的影响

顾泽玮, 单友田, 陈小雁, 蔡冬梅, 陈艳蝶, 景红娟

河南工业大学生物工程学院,郑州 450001

收稿日期:2018-06-29 出版日期:2018-12-10 发布日期:2019-12-18
通讯作者: 景红娟（1979-）,女,讲师,主要从事植物细胞自噬研究,（电子信箱）hjjing@haut.edu.cn。
作者简介:顾泽玮（1997-）,男,浙江舟山人,在读本科生,（电话）0371-67756513（电子信箱）guzewei@stu.haut.edu.cn
基金资助:
河南省科技厅自然科学项目（162300410175）; 河南工业大学小麦和玉米深加工国家工程实验室（NL2016011）; 河南工业大学 “省属高校基本科研业务费专项资金”项目（2016QNJH20）

Effects of Nitrate on Early Root Growth and Antioxidant Properties of Wheat Seedlings

GU Ze-wei, SHAN You-tian, CHEN Xiao-yan, CAI Dong-mei, CHEN Yan-die, JING Hong-juan

College of Biological Engineering,Henan University of Technology,Zhengzhou 450001,China

Received:2018-06-29 Online:2018-12-10 Published:2019-12-18

摘要/Abstract

摘要： 以小麦（Triticum aestivum L.）秋乐2122作为供试材料,以不含硝酸盐的Hoagland溶液为对照,研究不同浓度硝酸盐对小麦根系发育的影响。利用分光光度计和组化染色法检测根尖细胞中O₂^-·和H₂O₂含量,并测定细胞内相关氧化还原酶活性与还原型谷胱甘肽（GSH）含量。结果表明,硝酸盐能够显著增加小麦根尖细胞中O₂^-·而不是H₂O₂含量;高浓度硝酸盐（60 mmol/L）处理O₂^-·含量显著高于低浓度硝酸盐（10 mmol/L）处理。低浓度硝酸盐显著降低SOD酶活性和GSH含量,提高POD、谷胱甘肽还原酶（GR）和GSH-PX酶活性,使根部细胞具有良好的抗氧化能力,促进小麦根系生长。高浓度硝酸盐抑制小麦幼苗根系生长,是由于降低SOD与GSH-PX酶活性;虽然增加POD和GR酶活性,但是仍无法缓解根部细胞O₂^-·过量积累而造成的氧化损伤。所以不同浓度硝酸盐通过调节胞内O₂^-·含量,实现对小麦根系生长的调控。

关键词: 硝酸盐, 活性氧, 小麦（Triticum aestivum L.）, GSH, 根系

Abstract: In order to study the effects of different concentration nitrate on root growth of wheat（Triticum aestivum L.） seedlings,the nitrate-free Hoagland solution was used as a control and Qiule 2122 was as test material. O₂^-·,H₂O₂,glutathione（GSH） contents and oxidoreductase activity in roots were detected by pectrophotometry and histochemical staining. The results showed that nitrate significantly increased superoxied anion（O₂^-·） but not peroxide hydrogen（H₂O₂）,and content of O₂^-· in seedlings with high nitrate （60 mmol/L） was higher than that in wheat seedlings with low nitrate （10 mmol/L）. Superoxidase（SOD） activity and GSH content were significantly decreased by low nitrate. On the contrary,low nitrate increased POD,GR,and GSH-PX activities to maintaining higher antioxidant capacity. Thus,low nitrate activated the growth of the roots of wheat seedlings. High nitrate inhibited the root growth due to the decreasing activities of SOD and GSH-PX,although high nitrate increased activities of GR and POD. Therefore,nitrate regulated growth of roots by means of changing O₂^-· content.

Key words: nitrate, reactive oxygen, wheat（Triticum aestivum L.）, GSH, roots

中图分类号:

S512.1

顾泽玮, 单友田, 陈小雁, 蔡冬梅, 陈艳蝶, 景红娟. 硝酸盐对小麦幼苗根系早期生长和抗氧化特性的影响[J]. 湖北农业科学, 2018, 57(23): 61-64.

GU Ze-wei, SHAN You-tian, CHEN Xiao-yan, CAI Dong-mei, CHEN Yan-die, JING Hong-juan. Effects of Nitrate on Early Root Growth and Antioxidant Properties of Wheat Seedlings[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(23): 61-64.

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硝酸盐对小麦幼苗根系早期生长和抗氧化特性的影响

Effects of Nitrate on Early Root Growth and Antioxidant Properties of Wheat Seedlings

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