鮟鱇鱼子低分子质量肽的制备及其抗氧化活性评价

doi:10.14088/j.cnki.issn0439-8114.2022.23.030

湖北农业科学 ›› 2022, Vol. 61 ›› Issue (23): 150-154.doi: 10.14088/j.cnki.issn0439-8114.2022.23.030

鮟鱇鱼子低分子质量肽的制备及其抗氧化活性评价

任哲昕, 姚思佳, 叶韩薇, 陈媛媛, 唐云平

浙江海洋大学食品与药学学院/浙江省海洋生物医用制品工程技术研究中心,浙江舟山 316022

收稿日期:2021-12-27 出版日期:2022-12-10 发布日期:2023-01-27
通讯作者: 唐云平（1985-）,男,副研究员,博士,主要从事海洋活性肽的开发与利用,（电子信箱）tangyunping1985@163.com。
作者简介:任哲昕（1996-）,女,浙江杭州人,在读硕士研究生,研究方向为海洋活性肽的开发与利用,（电话）0580-2260600（电子信箱）renzhexin0802@163.com。
基金资助:
国家自然科学基金青年项目（41806153）; 浙江省新苗人才计划项目（2021R411034）

Preparation and antioxidant activity evaluation of low molecular mass peptides from monkfish （Lophius litulon） roe

REN Zhe-xin, YAO Si-jia, YE Han-wei, CHEN Yuan-yuan, TANG Yun-ping

School of Food and Pharmacy, Zhejiang Ocean University/Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, Zhoushan 316022,Zhejiang, China

Received:2021-12-27 Online:2022-12-10 Published:2023-01-27

摘要/Abstract

摘要： 以鮟鱇鱼（Lophius litulon）子为原料,利用复合酶解法制备低分子质量肽并对其抗氧化活性进行评价。利用胃蛋白酶和胰蛋白酶对鮟鱇鱼子进行酶解,超滤后得到分子质量小于1 kDa的鮟鱇鱼子肽（MRP）,并对其在体外的自由基（DPPH、ABTS及·OH）清除能力进行测定。此外,通过测定细胞活性、MDA、SOD、MDA和GSH-Px水平,研究其对H₂O₂诱导的RAW 264.7细胞损伤的保护作用。结果表明,MRP对DPPH、ABTS和·OH具有良好的清除作用,且能保护H₂O₂对RAW 264.7细胞的氧化损伤,可提高细胞活性及细胞中CAT、SOD、GSH-Px的活力,下调MDA的含量水平。结果表明,MRP具有较好的抗氧化能力,可作为添加剂用于抗氧化相关产品研发。

关键词: 鮟鱇鱼（Lophius litulon）子, 低分子质量肽, 过氧化氢, RAW 264.7细胞, 抗氧化

Abstract: The monkfish（Lophius litulon） roe was used as the raw material, the low molecular weight peptide was prepared by complex enzymatic hydrolysis and its antioxidant activity was evaluated. The monkfish roe was hydrolyzed by pepsin and trypsin, respectively. After ultrafiltration, the monkfish roe peptide （MRP） with molecular mass less than 1 kDa was obtained, and its free radicals （DPPH, ABTS, and ·OH） were detected in vitro. In addition, the protective effects of H₂O₂-induced RAW 264.7 cell damage were studied by measuring the cell vitality, MDA, SOD, MDA and GSH-Px levels. The results showed that MRP had a good scavenging effect on DPPH, ABTS and ·OH, and could protect the oxidative damage of RAW 264.7 cells from H₂O₂ to a certain extent. MRP could improve the cell activity and the activities of CAT, SOD and GSH-Px, and reduce the level of MDA. In conclusion, our results indicated that MRP had good antioxidant capacity and could be used as an additive in the research and development of anti-oxidation related products.

Key words: monkfish （Lophius litulon） roe, low molecular mass peptides, H₂O₂, RAW 264.7 cell, antioxidant

中图分类号:

TS201.2

任哲昕, 姚思佳, 叶韩薇, 陈媛媛, 唐云平. 鮟鱇鱼子低分子质量肽的制备及其抗氧化活性评价[J]. 湖北农业科学, 2022, 61(23): 150-154.

REN Zhe-xin, YAO Si-jia, YE Han-wei, CHEN Yuan-yuan, TANG Yun-ping. Preparation and antioxidant activity evaluation of low molecular mass peptides from monkfish （Lophius litulon） roe[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(23): 150-154.

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鮟鱇鱼子低分子质量肽的制备及其抗氧化活性评价

Preparation and antioxidant activity evaluation of low molecular mass peptides from monkfish （Lophius litulon） roe

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