基于糖基功能化磁性纳米颗粒材料富集-荧光法快速检测包装饮用水中铜绿假单胞菌

doi:10.14088/j.cnki.issn0439-8114.2025.02.027

湖北农业科学 ›› 2025, Vol. 64 ›› Issue (2): 171-178.doi: 10.14088/j.cnki.issn0439-8114.2025.02.027

基于糖基功能化磁性纳米颗粒材料富集-荧光法快速检测包装饮用水中铜绿假单胞菌

黄徽¹, 郭银利², 彭青枝¹, 鲁振坦², 王福燕³, 柳迪¹, 张苗¹

1.湖北省食品质量安全监督检验研究院/国家市场监管重点实验室（动物源性食品中重点化学危害物检测技术）,武汉 430075;
2.武汉纺织大学技术研究院,武汉 430200;
3.江汉大学医学部,武汉 430056

收稿日期:2023-10-13 出版日期:2025-02-25 发布日期:2025-03-07
通讯作者: 王福燕（1975-）,女,湖北江陵人,讲师,博士,主要从事公共卫生与安全研究,（电话）18971283408（电子信箱）1447004395@qq.com。
作者简介:黄徽（1989-）,男,湖北钟祥人,工程师,硕士,主要从事食品安全检测研究,（电话）15871439229（电子信箱）781312833@qq.com。

Rapid detection of Pseudomonas aeruginosa in packaged drinking water using glycosylation functionalized magnetic nanoparticle materials enrichment fluorescence method

HUANG Hui¹, GUO Yin-li², PENG Qing-zhi¹, LU Zhen-tan², WANG Fu-yan³, LIU Di¹, ZHANG Miao¹

1. Key Laboratory of Detection Technology of Focus Chemical Hazards in Animal-derived Food for State Market Regulation,Hubei Provincial Institute for Food Supervision and Test,Wuhan 430075, China;
2. Science & Technology Institute,Wuhan Textile University,Wuhan 430200,China;;
3. School of Medicine,Jianghan University,Wuhan 430056,China

Received:2023-10-13 Published:2025-02-25 Online:2025-03-07

摘要/Abstract

摘要： 基于铜绿假单胞菌（Pseudomonas aeruginosa）对糖基功能化磁性纳米颗粒材料特异性识别和铜绿假单胞菌分泌明胶酶的特点,诱导负载GNP@FITC功能化纳米纤维膜上FITC的释放,采用荧光分光光度计测定功能化纳米纤维膜的荧光强度,建立包装饮用水中铜绿假单胞菌的快速检测方法。该方法采用糖基功能化磁性纳米颗粒材料富集铜绿假单胞菌,250 mL饮用水中糖基功能化磁性纳米颗粒材料的添加量为5.00 mg,糖基功能化磁性纳米颗粒材料与铜绿假单胞菌的最佳共培养时间为6 h,负载GNP@FITC功能化纳米纤维膜与铜绿假单胞菌的最佳共培养时间为30 min,检验时间小于8 h,方法检出限为0.004 CFU/mL,特异性强。相较于GB 8538—2022《食品安全国家标准饮用天然矿泉水检验方法》中的检测方法,该方法缩短了检测时间,且检出限能够满足监管需要,可作为传统检测方法的有益补充。

关键词: 铜绿假单胞菌（Pseudomonas aeruginosa）, 糖基功能化, 磁性纳米颗粒材料, 富集, 荧光, 快速检测, 包装饮用水

Abstract: Based on the specific recognition of glycosylation functionalized magnetic nanoparticle materials by Pseudomonas aeruginosa and the secretion of gelatinase by Pseudomonas aeruginosa, the release of FITC was induced from load GNP@FITC functionalized nanofiber membrane. The fluorescence intensity of functionalized nanofiber membrane was measured using a fluorescence spectrophotometer to establish a rapid detection method for Pseudomonas aeruginosa in packaged drinking water. This method used glycosylation functionalized magnetic nanoparticle materials to enrich Pseudomonas aeruginosa. The amount of glycosylation functionalized magnetic nanoparticle materials added to 250 mL of drinking water was 5.00 mg, and the optimal co culture time between glycosylation functionalized magnetic nanoparticle materials and Pseudomonas aeruginosa was 6 hours. The optimal co-culture time between load GNP@FITC functionalized nanofiber membrane and Pseudomonas aeruginosa was 30 minutes, with a test time of less than 8 hours. The detection limit of the method was 0.004 CFU/mL, indicating strong specificity. Compared with the testing method in GB 8538—2022 ‘National Food Safety Standard for Drinking Natural Mineral Water Inspection Method’, this method shortened the testing time and the detection limit could meet regulatory needs, making it a useful supplement to traditional testing methods.

Key words: Pseudomonas aeruginosa, glycosylation functionalization, magnetic nanoparticle materials, enrichment, fluorescence, rapid detection, packaged drinking water

中图分类号:

TS207.3

黄徽, 郭银利, 彭青枝, 鲁振坦, 王福燕, 柳迪, 张苗. 基于糖基功能化磁性纳米颗粒材料富集-荧光法快速检测包装饮用水中铜绿假单胞菌[J]. 湖北农业科学, 2025, 64(2): 171-178.

HUANG Hui, GUO Yin-li, PENG Qing-zhi, LU Zhen-tan, WANG Fu-yan, LIU Di, ZHANG Miao. Rapid detection of Pseudomonas aeruginosa in packaged drinking water using glycosylation functionalized magnetic nanoparticle materials enrichment fluorescence method[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(2): 171-178.

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基于糖基功能化磁性纳米颗粒材料富集-荧光法快速检测包装饮用水中铜绿假单胞菌

Rapid detection of Pseudomonas aeruginosa in packaged drinking water using glycosylation functionalized magnetic nanoparticle materials enrichment fluorescence method

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