花魔芋交替氧化酶家族基因鉴定及表达分析

doi:10.14088/j.cnki.issn0439-8114.2025.12.039

湖北农业科学 ›› 2025, Vol. 64 ›› Issue (12): 235-243.doi: 10.14088/j.cnki.issn0439-8114.2025.12.039

花魔芋交替氧化酶家族基因鉴定及表达分析

关婉婷¹, 何斐², 罗昌新¹, 易倩¹, 赵孝杰¹, 濮兴艳¹, 孙柯欣¹, 朱凤艳¹, 褚洪龙¹

1.曲靖师范学院生物与食品工程学院,云南曲靖 655011;
2.安康学院现代农业与生物科技学院/富硒魔芋陕西省高校工程研究中心,陕西安康 725000

收稿日期:2025-08-11 发布日期:2025-12-30
通讯作者: 褚洪龙(1988-),男,山东微山人,副教授,博士,主要从事植物与微生物互作抗逆研究,（电话）15287808318（电子信箱）chuhonglo@163.com。
作者简介:关婉婷（2003-）,女,福建漳州人,在读硕士研究生,研究方向为魔芋资源保护与利用,（电话）18359607656（电子信箱）1107749012@qq.com。
基金资助:
国家自然科学基金（31860057）; 云南省基础研究专项面上项目（202401AT070002）; 陕西省科技厅项目(2025JC-YBQN-297; 2025NC-YBXM-378); 陕西省教工委项目(2023JCRC01); 曲靖师范学院创新研究团队资助项目

Identification and expression analysis of the alternative oxidase family gene in Amorphophallus konjac

GUAN Wan-ting¹, HE Fei², LUO Chang-xin¹, YI Qian¹, ZHAO Xiao-jie¹, PU Xing-yan¹, SUN Ke-xin¹, ZHU Feng-yan¹, CHU Hong-long¹

1. School of Biological and Food Engineering, Qujing Normal University, Qujing 655011, Yunnan, China;
2. College of Modern Agriculture and Biotechnology, Ankang University/Shaanxi University Engineering Research Center of High-Se-rich Konjac, Ankang 725000, Shaanxi, China

Received:2025-08-11 Online:2025-12-30

摘要/Abstract

摘要： 基于全基因组水平对花魔芋（Amorphophallus konjac K.Koch）的交替氧化酶（AOX）家族基因进行分析。结果表明,花魔芋共有4个AOX家族基因成员,命名为AkAOX1至AkAOX4,其蛋白长度为330~361 aa,分子质量为37 087.52~41 243.83 u,等电点为5.97~7.27,主要分布在细胞质和叶绿体上。花魔芋AOX基因家族成员分为2个亚族,分别是AOX1亚家族（AkAOX1、AkAOX2、AkAOX3）和AOX2亚家族（AkAOX4）。基因共线性分析表明,花魔芋无潜在的复制基因对,无串联重复事件;Ka/Ks分析进一步表明,这些同源基因对在进化过程中主要受到纯化选择。花魔芋AOX家族基因的启动子区域包含植物激素响应、生长发育响应和环境（胁迫）响应三类顺式作用元件。qRT-PCR结果显示,AkAOX1基因的表达量在软腐病病原菌侵染、干旱胁迫和盐胁迫下均显著上调,AkAOX4基因的表达量在软腐病病原菌侵染下显著下调,推测花魔芋AOX家族基因在其生长发育、激素信号传导及对生物和非生物胁迫的响应中均发挥重要作用。

关键词: 花魔芋（Amorphophallus konjac K.Koch）, 交替氧化酶（AOX）, 家族基因, 鉴定, 表达分析

Abstract: The alternative oxidase（AOX） family gene in Amorphophallus konjac K.Koch was analyzed at the whole-genome level. The results showed that there were four alternative oxidase family gene members in Amorphophallus konjac, named AkAOX1 to AkAOX4. Their protein length ranged from 330 to 361 aa, molecular weights from 37 087.52 to 41 243.83 u, and isoelectric points from 5.97 to 7.27. They were primarily located in the cytoplasm and chloroplast. The Amorphophallus konjac alternative oxidase family gene members were divided into two subfamilies, namely the AOX1 subfamily (AkAOX1, AkAOX2, AkAOX3) and the AOX2 subfamily (AkAOX4). Gene collinearity analysis indicated no potential duplicated gene pairs or tandem duplication events in Amorphophallus konjac. Ka/Ks analysis further showed that these homologous gene pairs were primarily under purifying selection during evolution. The promoter regions of the Amorphophallus konjac alternative oxidase family gene contained three types of cis-acting elements, associated with plant hormone response, growth and development response, and environmental (stress) response. The qRT-PCR results showed that the expression level of the AkAOX1 gene was significantly up-regulated under soft rot pathogen infection, drought stress, and salt stress, while the expression level of the AkAOX4 gene was significantly down-regulated under soft rot pathogen infection. It was speculated that the Amorphophallus konjac alternative oxidase family gene played important roles in its growth and development, hormone signal transduction, and responses to biotic and abiotic stresses.

Key words: Amorphophallus konjac K.Koch, alternative oxidase（AOX）, family gene, identification, expression analysis

中图分类号:

Q944.6

关婉婷, 何斐, 罗昌新, 易倩, 赵孝杰, 濮兴艳, 孙柯欣, 朱凤艳, 褚洪龙. 花魔芋交替氧化酶家族基因鉴定及表达分析[J]. 湖北农业科学, 2025, 64(12): 235-243.

GUAN Wan-ting, HE Fei, LUO Chang-xin, YI Qian, ZHAO Xiao-jie, PU Xing-yan, SUN Ke-xin, ZHU Feng-yan, CHU Hong-long. Identification and expression analysis of the alternative oxidase family gene in Amorphophallus konjac[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(12): 235-243.

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花魔芋交替氧化酶家族基因鉴定及表达分析

Identification and expression analysis of the alternative oxidase family gene in Amorphophallus konjac

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