Abstract: A UPLC-MS/MS method was developed and established to raplidly determine amantadine and rimantadine in honey. The sample were extracted with acetonitrile with 1% formic acid. After enrichment,the sample was separated by UPLC,eluted with acetonitrile and 0.1% formic acid as the mobile phase and the MRM reaction was monitored. The results showed that there was a good linearity in the range of 0.1~100 μg/L, and the correlation coefficient were more than 0.999. The detection limit of the amantadine was 0.2 μg/kg, and the limit of quantification was 0.5 μg/kg;the detection limit of the rimantadine was 0.5 μg/kg, and the limit of quantification was 1.0 μg/kg. The average recoveries were 75%~110% in the range from 1~20 μg/kg,and the precision was less than 20%. The method was convenient, rapid, accurate and it was suitable for the detection of amantadine and rimantadine in honey.

Key words: honey, amantadine, rimantadine, residues, UPLC-MS/MS