Determination of hyperuricemia drugs for treatment illegally added in Chinese patent medicines and health foods by UPLC-MS/MS

doi:10.14088/j.cnki.issn0439-8114.2020.11.026

Abstract

Abstract: To establish a method for simultaneous detection of 7 hyperuricemia drugs for treatment illegally added to Chinese patent medicines and health foods by UPLC-MS/MS．The samples were extracted from samples with mobile phase, the column was a ACQUITY UPLC® BEH C18（2.1 mm×100 mm, 1.7 μm）, a gradient elution with the mobile phase of 0.2% formic acid aqueous solution and acetonitrile solution at a flow rate of 0.3 mL/min. The compounds were detected by electrospray ion source in positive mode with multiple reaction monitoring （MRM） mode．The results showed that all the 7 hyperuricemia drugs for treatment with showed good linearity in their reasonable ranges , and the correlation coefficients were greater than 0.995.The average recoveries of low, medium and high concentrations ranged between 95.49% and 104.87%, and the relative standard deviations were 1.40%~3.44%. The LODs were 0.01~0.30 μg/kg and LOQs were 0.04~1.00 μg/kg.Illegal added chemical drugs were found in 1 of the 6 tested practical samples. With the advantages of simple pre-processed, high sensitivity and accurate results, the method can be used in determination of 7 hyperuricemia drugs for treatment in Chinese patent medicine and health foods.

Key words: UPLC-MS/MS, Chinese patent medicine, health food, illegal addition, hyperuricemia drugs

CLC Number:

O657.7⁺2

CHEN Yu, LU Sen-hua, PANG Xiao-lian, XU Meng, QIU Yi-xian. Determination of hyperuricemia drugs for treatment illegally added in Chinese patent medicines and health foods by UPLC-MS/MS[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(11): 125-130.

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