Isolation,Screening,Identification and Phosphate Solubilizing Ability of Phosphate Solubilizing Bacteria

doi:10.14088/j.cnki.issn0439-8114.2019.01.007

Abstract

Abstract: In order to develop an efficient microbial fertilizer solution, 11 strains from the farm-land soil of actinidia park in Zhouzhi county, Shaanxi prvince, were isolated, using phosphate volubility bacteria selective culture medium （Mongolia lecithin culture medium）. A strain of phosphate volubility bacteria JYP9 was screened through purification cultivar. And classification information of this strain was identified by using 16S rDNA gene sequence analysis, the results showed that the strain was a Pseudomonas extremorientalis. Then the phosphate solubilizing method and the liquid shake flask method were used in the experiment to confirm the optimum cultivation condition of JYP9 with lecithin as the only phosphorus source. The results showed that the optimal temperature was 26 ℃, optimal rotational was 200 r/min, optimal pH was 7, and optimum initial inoculation amount was 2%.

Key words: phosphate solubilizing bacteria, screen, identify, optimum condition

CLC Number:

S154.39

SHANGGUAN Yi-qing, CHANG Fan, LYU Rui, QI Fan, JIA Feng-an, WANG Yan, DING Hao. Isolation,Screening,Identification and Phosphate Solubilizing Ability of Phosphate Solubilizing Bacteria[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(1): 30-34.

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