Optimization of soil available selenium extraction conditions and bioavailability by response surface methodology

doi:10.14088/j.cnki.issn0439-8114.2019.15.025

Abstract

Abstract: Using soil as material, KH₂PO₄ solution was used to extract available selenium from soil. Taking the available selenium extraction amount as the response value, the extract concentration, pH value, extraction time and liquid sample ratio were independent variables, and the mathematical model was established. The optimal extraction conditions of soil available selenium were obtained by response surface methodology. The results showed that the optimal extraction conditions for soil available selenium wereextract concentration 0.5 mol/L, pH 9.9, extraction time 72 min, liquid sample ratio 27∶1, and the available selenium extraction amount was 0.56 mg/kg under the optimized conditions, which is basically consistent with the response surface analysis predicted value of 0.53 mg/kg. The crop pot experiment showed that the KH₂PO₄ solution extraction method for soil available selenium can be used as an index to evaluate the soil available for crop selenium.

Key words: soil, available selenium, response surface

CLC Number:

S151.9⁺3

LIU Shu-qin, CHEN Yong-bo, HUANG Guang-yu, XIONG Qian, ZHANG Chao-yang, CHEN E, QIN Bang, ZOU Xue-yuan. Optimization of soil available selenium extraction conditions and bioavailability by response surface methodology[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(15): 107-110.

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