Optimization of extraction technology and content determination of total flavonoids from Podocarpus macrophyllus （Thunb.） D. Don

doi:10.14088/j.cnki.issn0439-8114.2019.18.024

Abstract

Abstract: The total flavonoids of the stem of Podocarpus macrophyllus（Thunb.） D. Don was extracted by ultrasonic extraction, and the effects of extraction methods, solvent concentration, extraction sdvent volume and extraction time on total flavonoids were investigated by single factor test. Orthogonal experiment was used to optimize the extraction process and select the best extraction process of total flavonoids from the stem of Podocarpus macrophyllus（Thunb.） D. Don. The results showed that the best extraction technology was 70% ethanol, ultrasonic extraction of 1.0 h, and the ratio of material to liquid 1∶20（m/V）. The ultrasonic extraction method can be used to extract the total flavonoids of the stem of Podocarpus macrophyllus（Thunb.） D. Don. The method has good reproducibility, stability and feasibility.

Key words: Podocarpus macrophyllus（Thunb.） D. Don, stem, total flavonoids, extraction technology

CLC Number:

R282.2

WEI Jin-mei, LU Mei-yuan, WEI Xiu-mei, QIU Qin, HUANG Feng-yin, CHEN Ke-cheng, ZHEN Han-shen. Optimization of extraction technology and content determination of total flavonoids from Podocarpus macrophyllus （Thunb.） D. Don[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(18): 98-101.

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