A tri-primer multiplex PCR method for the detection of exogenous genes in the cry1Ab/cry1Ac transgenic rice TT51-1

doi:10.14088/j.cnki.issn0439-8114.2019.24.056

Abstract

Abstract: A tri-primer multiplex PCR method was established for the detection of cry1Ab/cry1Ac gene in the cry1Ab/cry1Ac transgenic rice TT51-1 and its backcross progenies of Huahui No.1. According to the foreign gene sequence inserted in TT51-1 and the rice genome sequence on the left and right sides of the insertion site, three PCR primers P1, P2 and P3 were designed. The results showed that the three primers PCR system could accurately distinguish the homozygous, heterozygous and negative genotypes of cry1Ab/cry1Ac gene. Only one 298 bp fragment was amplified in homozygote and only one 787 bp fragment was amplified in negative materials. Both 298 bp and 787 bp fragments were amplified from the heterozygote. The establishment of this system provides a simple and effective gene identification technique for molecular breeding of borer-resistant rice using TT51-1.

Key words: transgenic rice, multiplex PCR, cry1Ab/cry1Ac, TT51-1, Huahui No.1

CLC Number:

S511

ZHA Zhong-ping, WAN Bing-liang, YIN De-suo, DU Xue-shu, LI Jin-bo, XIA Ming-yuan, QI Hua-xiong, LI Yuan-meng. A tri-primer multiplex PCR method for the detection of exogenous genes in the cry1Ab/cry1Ac transgenic rice TT51-1[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(24): 232-235.

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