Abstract: ： In order to optimize the artificial cultivation techniques for clematis（Anoectochilus roxburghii）, orthogonal design experiments were used to study the effects of different cultivation substrates, different densities, and different fertilizers on the yield and effective components of A. roxburghii. The results showed that the cultivation substrate, planting density, and fertilizer had very significant effects on A. roxburghii polysaccharides, amino acids, and vitamin C; the cultivation substrate and planting density had significant effects on ash content; and the fertilizer had significant effects on Ca content. Synthesizing the effects of three factors on the effective components and economic yield of clematis, taking the cultivation substrate as peat soil∶coconut bran∶sand=4∶2∶1, cultivation density 3 cm×3 cm, and fertilizer management with No. 2 Feihuabao No.5 1 000 times solution+amino acid 1 000 times solution can promote economic benefits and quality improvement of clematis.

Key words: Anoectochilus roxburghii, cultivation substrate, planting density, yield, quality