The identification and sequence analysis of the core gene of Hippo pathway in wild silkworm

doi:10.14088/j.cnki.issn0439-8114.2020.13.034

Abstract

Abstract: The Hippo signaling pathway plays a key role in animal growth and development.According to the known sequences, we used BLAST software to identify the sequence of Hippo pathway component gene in the transcriptome of wild silkworm （Bombyx Mandarina）. The protein molecular weight, isoelectric point, secondary structure, conserved domain and conserved element were also prospected and analyzed by ORFinder, web CD-search tool, Expasy-protparam, EMBL-EBI and MEME bioinformatics tools. The homologous sequence alignment and phylogeny were analyzed by Clustalx and MEGA 6.0 software. The homologous genes of salvador, warts, mats, yorkie and scalloped were identified in wild silkworm, and ORF frame and coding protein were predicted. The result showed that,Warts, Sav and Mats of wild silkworm shared up than 98% similarity with that of domestic silkworm, while Yki and Scalloped shared 89.02% and 79.13% identity respectively between wild and domestic silkworm. The WW2 domain of BmYKi were deleted, and one peptide were inserted in YAP-binding domain of BmSd, while Sd of wild silkworm missing the hinge region between nuclear localization signal motif and proline-rich motif. Yki and Sd could be clustered into subgroup according to species, families and genera, wild and domestic silkworm were clustered into the same branch,suggesting that the homologous proteins could play species-based functional specificity as well as functional conservation. This study could provide a theoretical basis for further study on the mechanism of Hippo pathway in wild silkworm.

Key words: wild silkworm （Bombyx mandarina）, Hippo pathway, cluster analysis

CLC Number:

Q785

MENG Gang, PENG Yun-wu, WANG Rui-xian, CHU Qu, LIANG Jia-jun, LING Jun. The identification and sequence analysis of the core gene of Hippo pathway in wild silkworm[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(13): 149-153.

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