Optimization of enrichment and purification process of puerarin in the production of pueraria starch

doi:10.14088/j.cnki.issn0439-8114.2020.18.021

Abstract

Abstract: The method of puerarin enrichment and purification from water discharged by producing pueraria starch using macroporous resin as adsorption material was studied. The saturated capacity of dynamic adsorption （SCD） and elution rate （ER） of fifteen type of resin for total isoflavones of pueraria was investigated. The results indicated that the SCD for total isoflavones of pueraria was 39.41 mg/mL resin and the ER of resin was 91.42% eluted by 70% ethanol aqueous at 1.0 BV/h flow rate. And 4.7 g of crude isoflavones with the content of total isoflavones of 61.67% （UV spectrophotometry） and the content of puerarin of 31.80% （HPLC） could be recovered from 3 L discharge water. A high purity sample which contained 96.37% puerarin was obtained by 5% HCl hydrolysis and recrystallization, and recovery of puerarin was 15.91%. This was the best process optimization for puerarin enrichment and purification from water extracted pueraria starch.

Key words: pueraria, puerarin, enrichment and purification, macroporous adsorbent resin, total isoflavones

CLC Number:

R282

XU Meng-wei, XIAO Li, LI Yi-jin, DONG Xin-rong, LIU De-ming, ZHU Chi-ming. Optimization of enrichment and purification process of puerarin in the production of pueraria starch[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(18): 107-111.

References

[1] 马继兴. 神农本草经辑注 [M]. 北京: 人民卫生出版社, 1995.194.
[2] 国家药典委员会. 中华人民共和国药典(一部)[S].
[3] 石勇, 吴正奇. 葛根和葛根保健品的国内外研究进展[J].湖北工学院学报, 2000,15(1):52-55.
[4] 张雁, 张孝祺, 吴伟琪, 等. 葛根资源的开发利用[J].中国野生植物资源,2000,19(6):26-29.
[5] 李天星, 李新民. 中药葛根的研究进展[J].湖南中医杂志, 2013,29(8):151-153.
[6] 陈荔炟, 陈树和,刘焱文.葛根资源化学成分和药理作用研究概况[J].时珍国医国药,2006,17(11):2305-2306.
[7] 裴月湖, 娄红祥. 天然药物化学(第7版)[M].北京:人民卫生出版社,2016.183.
[8] BOUE S M,WIESE T E,NEHLS S,et al.Evaluation of the estrogenic effects of legume extracts containing phytoestrogens[J]. J Agric Food Chem,2003,51:2193-2199.
[9] 郑高利, 张信岳, 郑经伟. 葛根和葛根总异黄酮的雌激素样活性[J].中药材, 2002, 25(8):566-568.
[10] 刘葵, 张恩娟. 大豆异黄酮的药理作用及临床应用研究进展[J].中国药房, 2006, 17(1):67-69.
[11] 刘新, 林於. 愈风宁心片体外溶出度比较[J].中国中药杂志, 2001, 26(2):132-133.
[12] 赵晓莉, 崔小兵. HPLC法测定葛根素葡萄糖注射液中葛根素及3'-甲氧基葛根素含量[J].中国中药杂志, 2000, 25(7):413-415.
[13] 王靖, 吉民, 华维一, 等.葛根素研究进展[J].药学进展, 2003, 27(2):70-73.
[14] 常虹, 周家华, 兰彦平, 等. 葛根淀粉提取工艺研究[J].现代食品科技, 2009, 25(5):523-526.
[15] 孙庆杰, 杨明毅. 葛根淀粉连续化生产工艺的研究[J].食品科学, 2002, 23(4): 93-94.
[16] 李侠,臧学丽,徐祎博,等. AB-8大孔树脂纯化绿豆皮黄酮工艺优化及纯化前后抗氧化能力比较[J]. 食品科学,2018, 39(10):283-290.