Abstract: To better understand the impact of climate change on Trabala vishnou gigantina Yang, the activities of two detoxification enzymes [glutathione S-transferase （GST）, acetylcholinesterase （AchE）] and three protective enzymes [catalase （CAT）, peroxidase （POD） and superoxide dismutase （SOD）] were determined and analyzed under normal CO₂ concentration （375 μL/L） and high CO₂ concentration （750 μL/L）. The results showed that the activities of the two detoxification enzymes in the larvae of different instars of Trabala vishnou gigantina Yang showed an increasing trend under high CO₂ concentration, the activity of GST was higher than that under normal CO₂ concentration, and the activities of GST in the larvae of the first, fifth and sixth instars were significantly different from those under the normal CO₂ concentration; under high CO₂ concentration, AchE activity of the 3rd to 6th instars was increased, and AchE activity of the 5th and 6th instars was significantly higher than that of the corresponding instars under normal CO₂ concentration. Under high CO₂ concentration, the changes of the three protective enzymes in the larvae of different instars of Trabala vishnou gigantina Yang were inconsistent with each other, and the activity of CAT showed an increasing trend, which was significantly higher than that of the corresponding instars under normal CO₂ concentration; the POD activity increased first and then decreased with the increase of instar, and there was no significant change of POD activity in the first instar, second instar and sixth instar larvae; the SOD activity of the larvae decreased with the increase of instars, which was significantly lower in the 4th, 5th and 6th instars than that of the corresponding instars under normal CO₂ concentration.

Key words: Trabala vishnou gigantina Yang, CO₂ concentration, protective enzyme, detoxification enzyme, activity