Development and validation of a tightly linked KASP marker for the rice brown planthopper resistance gene Bph43

doi:10.14088/j.cnki.issn0439-8114.2023.06.031

Abstract

Abstract: To promote the application of Bph43 in the breeding of rice varieties resistant to brown planthopper,on the basis of fine mapping of Bph43 in the early stage, the Bph43 gene donor parent IRGC 8678 was resequenced. By conducting SNP mutation site analysis with the Japanese reference genome and 3 000 rice sequencing public database resources, the study designed and developed Bph43 tightly linked interval specific KASP markers （K_11674982, K_11775428, and K_11856768）. The validation results of 66 natural populations of rice and 200 BC₁F₂ hybrid progeny showed that the three developed KASP markers were codominance markers, and the genotype analysis results of the markers were completely consistent with the phenotype of brown planthopper resistance, which could specifically and accurately detect whether there was Bph43 gene in rice. The Bph43 KASP marker developed based on SNP loci could be used to explore new germplasm carrying the Bph43 gene and molecular marker assisted selection breeding for resistance to brown planthopper.

Key words: rice, brown planthopper（Nilaparvata lugens）, Bph43, KASP marker, breeding for resistance to brown planthopper

CLC Number:

S435

DENG Zhao, WANG Kai, DU Bo, ZHU Li-li, YANG Yuan-zhu, CHEN Rong-zhi. Development and validation of a tightly linked KASP marker for the rice brown planthopper resistance gene Bph43[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(6): 169-174.

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