Effect of nitrogen application rate on chlorophyll content and photosynthetic characteristics of Leymus chinensis

doi:10.14088/j.cnki.issn0439-8114.2024.11.024

Abstract

Abstract: In order to explore the response of nitrogen application rate to chlorophyll content and photosynthetic characteristics of Leymus chinensis, five nitrogen （pure N） gradients （0, 75, 150, 225, 300 kg/hm²） were set in a potted experiment. Chlorophyll content and photosynthetic characteristics of L. chinensis were measured at the jointing stage. The effects of different nitrogen application rates on biomass, chlorophyll content and photosynthetic characteristics of L. chinensis were analyzed. The results showed as follows：①Compared with no nitrogen application, nitrogen application rate had a certain promotion effect on the total biomass of L. chinensis in the range of 75~150 kg/hm², and the most obvious promotion effect was at 150 kg/hm², which increased by 18.05%; ②the contents of chlorophyll a and carotenoids increased first and then decreased with the increase of nitrogen application rate, and reached the maximum values at 150 kg/hm², which were 1.68 mg/g and 0.64 mg/g, respectively; ③photosynthetic characteristics （net photosynthetic rate, stomatal conductance and transpiration rate） of L. chinensis generally increased first and then decreased with the increase of nitrogen application rate, and reached the maximum under N150 treatment. It was concluded that 150 kg/hm² nitrogen fertilizer had the best chlorophyll content, which promoted photosynthesis and further increased biomass accumulation of L. chinensis.

Key words: nitrogen, Leymus chinensis, biomass, chlorophyll, photosynthetic parameter

CLC Number:

S543⁺.9

QIU Chun-chen, HU Yu-tong, YAN Han, LI Hui-jun, YU Hao-nan, ZHAO Ting-ting, ZHOU Xiao-guo. Effect of nitrogen application rate on chlorophyll content and photosynthetic characteristics of Leymus chinensis[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(11): 141-146.

References

[1] 王红静, 马金宝, 丛百明, 等. 氮添加对科尔沁沙地羊草产量及氮肥利用效率的影响[J]. 草原与草坪, 2021, 41(2): 47-52.
[2] LIU G, QI D, DONG X, et al.Sheepgrass(Leymus chinensis): An environmentally friendly native grass for animals[J]. DOI:10.1007/978-981-13-8633-6.
[3] HUANG L,LIANG Z,SUAREZ D L,et al.Effects of continuous nitrogen application on seed yield, yield components and nitrogen-use efficiency of Leymus chinensis in two different saline-sodic soils of Northeast China[J]. Crop and pasture science,2019,70(4): 373-383.
[4] BAI X, CHENG J H, ZHENG S X, et al.Ecophysiological responses of Leymus chinensis to nitrogen and phosphorus additions in a typical steppe[J]. Chinese journal of plant ecology, 2014, 38(2): 103.
[5] 马军花, 武兰芳, 陈浩. 减量施肥对土壤可溶性有机碳氮组分的影响[J]. 种子科技, 2021, 39(17): 30-31.
[6] 李怀胜,艾海峰, 王贺亚, 等. 减量施肥对滴灌冬小麦生长、产量及养分利用的影响[J]. 新疆农业科学, 2022, 59(2): 329-336.
[7] 焉莉. 不同施肥管理对东北玉米连作地农业面源污染影响研究[D]. 长春: 吉林大学, 2016.
[8] XU Z Z, ZHOU G S.Combined effects of water stDress and high temperature on photosynthesis, nitrogen metabolism and lipid peroxidation of a perennial grass Leymus chinensis[J]. Planta, 2006, 224(5): 1080-1090.
[9] 才源, 李子勇, 于新海, 等. 适量氮肥对吉林西部人工建植羊草草原的种子产能与牧草品质影响的研究[J]. 农业与技术, 2022, 42(1): 16-20.
[10] 万东莉, 侯向阳, 丁勇, 等. 磷胁迫下羊草的响应及磷响应相关基因表达分析[J]. 中国农业科学, 2019, 52(23): 4215-4227.
[11] 单立文,张强,朱瑞芬,等. 氮、磷添加下AMF对羊草和苜蓿生长与光合生理特性的影响[J]. 草业学报,2020,29(8):46-57.
[12] 潘多锋, 张瑞博, 李道明, 等. 施氮期和收获期对羊草种子产量及质量的影响[J]. 草业科学, 2019, 36(8): 2078-2086.
[13] 卜冬冬,李昂,王银柳,等. 氮素和植物生长促进剂对羊草生长及竞争力的影响[J].应用生态学报,2019,30(8):2667-2674.
[14] 梁敏,杨瑞霞,许兴,等. 施肥对羊草分蘖及养分的影响[J]. 湖北农业科学, 2019, 58(3): 91-95.
[15] 赵丹丹, 马红媛, 李阳, 等. 水分和养分添加对羊草功能性状和地上生物量的影响[J]. 植物生态学报, 2019, 43(6): 501-511.
[16] 李文晶,王俊锋,高晓荻,等. 干旱条件下氮磷添加对羊草干草产量及饲用品质的影响[J]. 草地学报, 2021, 29(4): 743-748.
[17] 鲍士旦. 土壤农化分析[M]. 北京:中国农业出版社,2000.30-109.
[18] 邹琦. 植物生理学实验指导[M]. 北京: 中国农业出版社, 2000. 72-75.
[19] 王丽萍, 张凯, 贾宏涛, 等. 生物炭对原油污染土壤中苏丹草光合生理特征的影响[J]. 新疆农业大学学报, 2019, 42(1): 59-64.
[20] 张晓琳, 翟鹏辉, 黄建辉. 降水和氮沉降对内蒙古半干旱草地羊草和大针茅叶片碳交换的影响[J]. 草地学报, 2019, 27(4): 977-986.
[21] WALI M, GUNSE B, LIUGANY M, et al.High salinity helps the halophyte Sesuvium portulacastrum in defense against Cd toxicity by maintaining redox balance and photosynthesis[J]. Planta, 2016, 244(2): 333-46.
[22] 段祥光, 张利霞, 刘伟, 等. 施氮量对油用牡丹‘凤丹’光合特性及产量的影响[J]. 南京林业大学学报(自然科学版), 2018, 42(1):48-54.
[23] 赵浩波, 任卫波, 于秀敏, 等. 不同氮素水平对羊草形态及生理指标的影响[J].中国草地学报, 2020, 42(3): 15-20.
[24] 董晓兵,郝明德,郭胜安,等. 施肥对羊草产量和品质的影响[J]. 草业科学, 2014, 31(10): 1935-1942.
[25] 盖甜甜, 邹中华, 任志华, 等. 不同氮养分条件下油茶幼苗生物量及养分利用对磷水平的响应[J]. 江西农业大学学报, 2022, 44(3): 626-634.
[26] 吴小娟, 杨雯, 解如琳, 等. 高温胁迫对玉米品种‘郑单958’幼苗生长及部分生理指标的影响[J]. 植物资源与环境学报, 2018, 27(2): 117-119.
[27] 林荣呈, 杨文强, 王柏臣, 等. 光合作用研究若干前沿进展与展望[J]. 中国科学:生命科学, 2021, 51(10): 1376-1384.
[28] 胡洋,丛孟菲,张梦瑶,等. 土壤铅污染对糜子生长和光合生理特征的影响[J]. 新疆农业大学学报, 2020, 43(2): 150-156.
[29] 徐峰, 钟文, 胡昕, 等. 秸秆还田配施不同用量氮肥对小麦光合特性·产量及土壤养分的影响[J]. 安徽农业科学, 2022, 50(1): 160-162.
[30] 曲晓莲,李耕,仲锦维,等. 尿素类型与施肥深度对冬小麦花后旗叶光合特性与产量的影响[J]. 山东农业科学,2021,53(12):88-95.
[31] CROFT H, CHEN J M, LUO X, et al.Leaf chlorophyl content as a proxy for leaf photosynthetic capacity[J]. Glob chang biology, 2017, 23(9): 3513-3524.
[32] 马昕. 施氮对玉米穗期高温胁迫下穗位叶生理及产量的影响[D].郑州:河南农业大学,2022.
[33] 杨军, 陈小荣, 朱昌兰, 等. 氮肥和高温对早稻淦鑫203产量、SPAD值及可溶性糖含量的影响[J]. 江西农业大学学报, 2015, 37(5): 759-764.