The inhibitory effects of volatile oil of Sarcandra glabra on the Didymella glomerata of Sophora tonkinensis

doi:10.14088/j.cnki.issn0439-8114.2025.02.014

Abstract

Abstract: The antibacterial activity and mechanism of the volatile oil of Sarcandra glabra （Thunb.） Nakai against Didymella glomerata of Sophora tonkinensis Gagnep. were investigated. The inhibitory effects of Sarcandra glabra oil on the growth of Didymella glomerata were studied by measuring the colony diameter and mycelium weight. The electrical conductivity, soluble protein, reducing sugar and malondialdehyde （MDA） were determined to investigate the action mechanism of Sarcandra glabra oil. The results showed that the volatile oil of Sarcandra glabra had significant antibacterial activity against the Didymella glomerata of Sophora tonkinensis, and the EC₅₀ was 271.00 mg/L. The inhibitory effect on mycelial growth was the best when the concentration of volatile oil was 2 000 mg/L and the treatment time was 72 h. The inhibitory rate of colony expansion and mycelial growth were 100% and 95.00%, respectively. The electrical conductivity, soluble protein content and MDA content of the treatment group were significantly higher than those of the control group. The electrical conductivity and soluble protein content of the treatment were 1.54 times and 4.74 times of those the control, respectively when the mass concentration of volatile oil was 2 000 mg/L for 72 h. MDA reached its maximum value at 96 h, which was 3.11 times that of the control. The reducing sugar content in the treatment did not change much, and the reducing sugar content in the control group decreased with the extension of time, and was 53.36% lower than that in the treatment group at 72 h.

Key words: Sarcandra glabra （Thunb.） Nakai, Sophora tonkinensis Gagnep., volatile oil, Didymella glomerata, inhibitory effects

CLC Number:

QIU Zhuo-qiu, JIANG Ni, ZHAN Xin-jie, SONG Li-sha, LI Lin-xuan, HUANG Qi. The inhibitory effects of volatile oil of Sarcandra glabra on the Didymella glomerata of Sophora tonkinensis[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(2): 89-93.

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