The screening and biological characteristics of endophytic fungi from Hedyotis diffusa Willd. against citrus anthracnose pathogen

doi:10.14088/j.cnki.issn0439-8114.2025.02.019

Abstract

Abstract: The endophytic fungi of Hedyotis diffusa were isolated by the tissue block isolation method, and the strains with antagonistic activity against the citrus anthracnose pathogen were screened by the plate stand-off method. Finally, the screened antagonistic strains were identified and their molecular biological characteristics were studied. The results showed that the roots had the highest number of endophytic fungi isolated from Hedyotis diffusa, followed by the leaves and the stems had the least. Five of the 19 strains of endophytic fungi isolated from Hedyotis diffusa had a more pronounced inhibitory effect on pathogenic fungi. The BG10 strain was the most effective in antagonising the pathogen. Using molecular biotechnology （ITS）,BG10 was identified as Curvularia, presumably Curvularia lunata. Its optimal medium was Gao’s medium, the best carbon source was glucose and the best nitrogen source was beef paste.

Key words: Hedyotis diffusa Willd., endophytic fungi, citrus anthracnose pathogen, screening, biological characteristics

CLC Number:

ZHU Hua, HUANG Shi-ping, HUANG Qing-lin, ZHANG Miao, CHEN Long. The screening and biological characteristics of endophytic fungi from Hedyotis diffusa Willd. against citrus anthracnose pathogen[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(2): 121-127.

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