Isolation of endophytic fungi from Pholidota chinensis Lindl. and screening and identification of antibacterial strains

doi:10.14088/j.cnki.issn0439-8114.2025.02.018

Abstract

Abstract: Endophytic fungi strains with antifungal activity against pathogenic bacterial were isolated from the traditional medicinal plant Pholidota chinensis, and the antifungal activity of the strains was studied. Endophytic fungi of Pholidota chinensis were isolated by tissue block method and fungal inhibition test was carried out by paper method, and the strains with antibacterial activity were screened out. According to their morphological characteristics, the active strains with antibacterial activity were screened for DNA extraction and identification. The results showed that 49 endophytic fungi were isolated from the roots, rhizomes, leaves and pseudobulb of Pholidota chinensis, including 37 endophytic fungi from Hechi City, Guangxi and 12 endophytic fungi from Zhangzhou City, Fujian Province. Endophytic fungi HCY-5, HCY-9, HCY-10 isolated from the leaf tissues of Pholidota chinensis in Hechi City, Guangxi Province, and endophytic fungi FJY-2, FJY-5, FJY-6, FJY-7 isolated from Pholidota chinensis in Zhangzhou City, Fujian Province, showed good antibacterial activity against Staphylococcus aureus. Combined with morphological characteristics, the gene sequences of endophytic fungi strains were introduced into GenBank, and after comparison, the endophytic fungi HCY-5, HCY-9, HCY-10 in Hechi City, Guangxi and FJY-2, FJY-5, FJY-6, FJY-7 in Zhangzhou City, Fujian Province were identified as Pholiosticta, which was supposed to be Pholiosticta capitalensis. Endophytic fungi HCY-5, HCY-9, HCY-10 from Hechi City, Guangxi and endophytic fungi FJY-2, FJY-5, FJY-6 and FJY-7 from Zhangzhou City, Fujian Province had good antibacterial activity.

Key words: Pholidota chinensis Lindl., endophytic fungi, antibacterial activity, screening, identification

CLC Number:

ZHU Hua, WU Wen-yuan, ZHANG Miao, CHEN Long, YANG Li-rong, LIANG Ren-tao, HUANG Shi-ping, WU Huan, SHI Xiao-guang. Isolation of endophytic fungi from Pholidota chinensis Lindl. and screening and identification of antibacterial strains[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(2): 112-120.

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