Screening of α-glucosidase inhibitors in Eugenia caryophyllata and Cinnamomum cassia

doi:10.14088/j.cnki.issn0439-8114.2025.03.017

Abstract

Abstract: The purpose of this study was to explore the potential constituents responsible for α-glucosidase inhibitory activity of medicine food homologous herbs Eugenia caryophyllata and Cinnamomum cassia. The α-glucosidase inhibitory effect of crude extracts from Eugenia caryophyllata and Cinnamomum cassia were preliminary determined by in vitro enzyme activity experiment. The corresponding bioactive constituents were determined by the combined use of high-resolution inhibition profiling and ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS). The results showed that, crude methanol extracts of Eugenia caryophyllata and Cinnamomum cassia displayed considerable inhibition towards α-glucosidase with IC₅₀values of (50.0±0.08) μg/mL and (2.19±0.17) μg/mL, respectively, more active than the positive control acarbose [IC₅₀=(264.37±2.73) μg/mL]. The high-resolution inhibition profiles pointed out that polyphenols were correlated with α-glucosidase inhibitory activity. In addition, flavonoids from Eugenia caryophyllata, cinnamic acid and 2′-methoxy cinnamaldehyde from Cinnamomum cassia were considered as the possible inhibitors by the careful analysis of UPLC-QTOF-MS/MS data. The results supported further research with the aim of developing Eugenia caryophyllata and Cinnamomum cassia into functional food for prevention and management of T2D and T2D-related complications.

Key words: medicine food homologous herbs, α-glucosidase, high-resolution inhibition profiling, ultra high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS)

CLC Number:

R284.1

XU Cong. Screening of α-glucosidase inhibitors in Eugenia caryophyllata and Cinnamomum cassia[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(3): 107-111.

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