Detection of quality and resistance genes of wheat varieties (lines) in Huang-Huai wheat region by KASP genotyping technology

doi:10.14088/j.cnki.issn0439-8114.2025.04.010

Abstract

Abstract: To elucidate the genetic basis of quality and resistance traits in the dominant wheat （Triticum aestivum L.） varieties from the Huang-Huai wheat region, 99 wheat varieties (lines) were genotyped using 12 KASP markers related to quality, disease resistance, drought resistance and pre-harvest sprouting resistance. The results revealed that 33 materials carried the Glu-D1d gene encoding high-quality gluten subunit 5+10, and 39 materials contained the Glu-A1 gene encoding Ax1/Ax2* strong-gluten haplotype. In terms of disease resistance, only 12 materials exhibited Lr68, a leaf rust resistance gene, and none of the tested materials were found to carry the Fhb1 gene for Fusarium head blight resistantce. For sprout resistance, the haplotypes TaSdr-B1a, Rio Blanco type, and Hap-H associated with pre-harvest sprouting were detected in 9, 73, and 13 materials, respectively. In drought resistance, there were 53 materials carried 3Ba drought resistant haplotype of the COMT-3B gene, 45 materials possessed the TaDREB-B1a drought resistance haplotype, and 89 materials carried the A2a drought resistance haplotype of the TaSST-4A gene. It could be seen that the drought resistance genes detected in wheat varieties in Huang-Huai wheat region were widely distributed and accounted for a relatively high proportion, while the excellent haplotypes in disease resistance accounted for a relatively small proportion. Therefore, it was urgent to strengthen the introduction and utilization of disease resistance genes in breeding strategies.

Key words: wheat(Triticum aestivum L.), quality, disease resistance, drought resistance, pre-harvest sprouting resistance, gene detection, KASP genotyping technology, Huang-Huai wheat region

CLC Number:

S511
S336

WANG Zhi, LI Han-bing, WANG Wei, ZHANG Yu-jie, TIAN Mi, WANG Wei-wei. Detection of quality and resistance genes of wheat varieties (lines) in Huang-Huai wheat region by KASP genotyping technology[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(4): 51-57.

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