Abstract: In order to screen excellent wheat（Triticum aestivum L.） germplasm resources resistant to Fusarium head blight (FHB) , the FHB resistance levels of 60 wheat varieties (lines) including the control varieties were evaluated using single-floret injection and soil-surface inoculation methods. Sumai 3 (highly resistant, HR), Yangmai 158 (moderately resistant, MR), and Annong 8455 (highly susceptible, HS) served as control varieties. A total of 2 high-resistant varieties, 7 medium-resistant varieties, 30 medium-susceptible varieties and 21 high-susceptible varieties were screened out. Furthermore, the agronomic traits of wheat varieties (lines) with resistance levels of moderately susceptible (MS) and above were investigated. A total of 16 elite wheat varieties (lines) with superior agronomic traits and resistance to Fusarium head blight, such as Nei 4103, Chuanmai 45, W2, and Xinong 9871, were screened out, which provided reliable parental sources for new cultivars breeding.

Key words: wheat（Triticum aestivum L.）, varieties (lines), Fusarium head blight, resistance identification, single-floret injection methods, soil-surface inoculation methods