Effects of Various Food Ratio on the Development and the Activities of Digestive and Protective Enzymes of Tenebrio molitor Linnaens Larvae

doi:10.14088/j.cnki.issn0439-8114.2018.12.015

Abstract

Abstract: In order to understand the physiological adaptability of Tenebrio molitor larvae to different food proportions, the effects of five different food comparisons on the growth characteristics and the activities of digestive enzymes and protective enzymes in Tenebrio molitor larvae were determined. The results showed that different food comparisons had a significant effect on the development, digestive enzyme and protective enzyme activities of T. molitor larvae. Adding green fodder with high water content in wheat bran, especially high sugar content of apples, pears and other fruits were conducive to promoting the growth of T. molitor larvae. There was a positive correlation between the development and the protease activity of T. molitor larvae in different food proportions. The growth rate of the larvae of T. molitor was not correlated with the activity of protective enzymes, but the activity of SOD and CAT was the lowest in the larvae with the fastest growth of the treatment 5（83% wheat bran with 17% pear）.

Key words: Tenebrio molitor larvae, food proportions, development, digestive enzyme, protective enzyme

CLC Number:

S899.9

WANG Rui-rui, FU Bai-wen, WU Guang, YANG Zhen-de, HUANG Mei, FEI Ling-ling, LIN Ye-bang. Effects of Various Food Ratio on the Development and the Activities of Digestive and Protective Enzymes of Tenebrio molitor Linnaens Larvae[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(12): 53-56.

References

[1] 张生芳. 中国储藏甲虫[M].北京:中国农业科技出版社,1996.
[2] 杜开书,柴立英.黄粉虫饲养条件的研究现状与利用前景[J].安徽农业科学,2006,34(18):4646-4647.
[3] 谢保令. 黄粉虫营养成分的分析研究[J].昆虫知识,1994,31(3):175-176.
[4] 王春清,马铭龙,丁秀文,等.不同比例麦麸和玉米秸秆对黄粉幼虫生长性能的影响[J].中国畜牧兽医,2013,40(1):210-212.
[5] 郑华英,丁秀凤,解春霞,等.替代寄主繁育和饲料饲养对花绒寄甲成虫产卵及存活的影响[J].江苏林业科技,2016,43(2):13-16.
[6] 黄琼,周祖基,杨伟,等.繁育川硬皮肿腿蜂替代寄主的筛选[J].昆虫学报,2005,48(3):375-379.
[7] 钦俊德,王琛柱.论昆虫与植物的相互作用和进化的关系[J].昆虫学报,2001,44(3):360-365.
[8] 王倩倩,王蕾,李克斌,等.不同寄主植物对草地螟的营养作用及消化酶的影响[J].植物保护,2015,41(4):46-51.
[9] 胡登乾,何勇,姜金仲,等.不同饲料对黄粉虫成虫产卵量的影响[J].安徽农业科学,2016,44(1):93-94.
[10] 彭中健,黄秉资.黄粉虫的研究[J].昆虫知识,1993,30(2):111-115.
[11] 赵洪霞. 温度、光周期对绿盲蝽发育增殖的影响与取食不同寄主后体内消化酶活性差异[D].南京:南京农业大学,2011.
[12] 杨振德,朱麟,赵博光,等.苦豆草生物碱对分月扇舟蛾体内消化酶和保护酶活性的影响[J].北京林业大学学报,2006,28(1):80-83.
[13] 尹姣,冯红林,李克斌,等.寄主植物对草地螟中肠解毒酶及保护性酶活性的影响[J].植物保护,2012,38(1):35-39.
[14] 周琼,魏美才,欧晓明.环氧苍耳素Ⅰ对菜青虫中肠消化酶和羧酸酯酶活性的影响[J].昆虫学报,2011,54(6):729-733.
[15] 蒋传葵,金承德,吴仁龙,等.工具酶的活力测定[M].上海:上海科学技术出版社,1982.
[16] 朱俭,曹凯鸣,周润琦,等.生物化学实验[M].上海:上海科学技术出版社,1981.
[17] 李周直,沈惠娟,蒋巧根,等.几种昆虫体内保护酶系统活力的研究[J].昆虫学报,1994,37(4):399-403.
[18] 唐维媛,邢丛丛,董永刚,等.斜纹夜蛾幼虫感染莱氏野村菌后的抗氧化酶活性变化[J].昆虫学报,2015,58(5):526-534.
[19] SIMON L M,FATRAI Z,JONAS D E,et al.Study of peroxide metabolism enzymes during the development of Phaseolus vulgris[J].Biochemistry and Physiology of Pflanzen,1974,166(5-6):387-392.
[20] 陈毓荃. 生物化学实验方法和技术[M].北京:科学出版社,2002.
[21] 曹凤勤,崔志富,刘万学,等.螺旋粉虱对不同甜椒品种的产卵选择性及与植物中化学物质的相互关系研究[J].植物保护,2011,37(5):77-80.
[22] 钦俊德. 诠释植食性昆虫是怎样选择食料植物的[J].生物学通报,2003,38(6):1-3.
[23] HÓDAR J A,ZAMORA R,CASTRO J. Host utilisation by moth and larval survival of pine processionary caterpillar Thaumetopoea pityocampa in relation to food quality in three Pinus species[J].Ecol Entomol,2006,27(3):292-301.
[24] 夏淑春,王学武,孙丽娟,等.几种饲料对黄粉虫幼虫生长发育的影响[J].湖北农业科学,2013,52(17):4117-4122.
[25] 杨兆芬,倪明,黄敏,等.黄粉虫成虫繁殖力及影响幼虫发育的因素[J].昆虫知识,1999,36(1):24-27.
[26] 陈光道,王鹤,栾雪,等.不同饲养条件对黄粉虫幼虫生长发育的影响[J].中国林副特产,2017(1):22-23.
[27] 刘征涛. 不同食料对小地老虎生长发育与繁殖的影响[J].江西植保,1988(2):9-11.
[28] 张娜,郭建英,万方浩,等.寄主植物对甜菜夜蛾生长发育和消化酶活性的影响[J].植物保护学报,2009,36(2):146-150.
[29] 苏超. 寄主植物和温度对三条橙灯蛾Lemyra alikangensis取食及生长发育影响的研究[D].上海:华东师范大学,2014.
[30] 周奋启,陆艳艳,姚远,等.不同寄主植物对B型烟粉虱种群保护酶和解毒酶的影响[J].江苏农业学报,2011,27(1):57-61.