Improvement of Rice Blast Resistance in Red Rice by Pyramiding of Pi1 and Pi2 through Molecular Marker-Assisted Selection

doi:10.14088/j.cnki.issn0439-8114.2018.18.005

Abstract

Abstract: Red rice is an extremely precious characteristic rice resource. Blast is one of the most serious rice diseases worldwide,which usually leads to a sharp decline in rice yield and even results in no yield. Breeding blast-resistant varieties is one of the most effective and economical ways to control the disease. In this study,BL122 containing two blast resistance genes Pi1 and Pi2 was used as gene donor to be crossed and then backcrossed with red rice variety Guihong 1. The improved materials of high resistance to rice blast with two resistance genes were obtained. The resistance identification of rice blast in seedling stage and mature stage of BC₃F₃ was carried out. The results showed that the resistance of target strains with two resistance genes was all above the resistance level,and some strains reached high resistance level. The resistance of these strains to rice blast was significantly higher than that of the recurrent parent. Statistical analysis showed that,compared with the control,the effective panicle and theoretical yield of BC₃F₃ were significantly increased, while there was no significant difference in other agronomic traits. The improved materials obtained through molecular marker-assisted selection is a new breeding resource for the breeding of red rice.

Key words: red rice, molecular marker-assisted selection, rice blast, gene pyramiding, Pi1, Pi2

CLC Number:

SUN Fu, TANG Mei, HE Cong, LU Hong-cong. Improvement of Rice Blast Resistance in Red Rice by Pyramiding of Pi1 and Pi2 through Molecular Marker-Assisted Selection[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(18): 23-27.

References

[1] 王丽华,叶小英,李杰勤,等.黑米、红米的营养保健功效及其色素遗传机制的研究进展[J].种子,2006,25(5):50-53.
[2] MA J,CHEN Q X,LING W H.Study of red and black rice to health care effects[J].Food Science,2000,21(12):139-140.
[3] 朱建清. 水稻糙米色素、育性遗传,基因定位及稻粒黑粉菌侵染水稻的组织病理学研究与防治[D].成都:四川大学,2004.
[4] SHEN Y,HENRY A,ZHU X D,et al.Evaluation of resistance of some hybrid rice,conventional early indica and late japonica rice to Magnaporthe grisea in China[J].Scientia Agricultura Sinica,2004,37(3):362-369.
[5] LIU B,ZHANG S,ZHU X,YANG Q,et al.Candidate defense genes as predictors of quantitativeblast resistance in rice[J].Molecular Plant-Microbe Interactions,2004,17(10):1146-1152.
[6] YU Z H,MACKILL D J,BONMAN J M,et al.Molecular mapping of genes for resistance to rice blast(Pyricularia grisea Sacc.)[J].Theor Appl Genet,1996,93:859-863.
[7] YU Z H,MACKILL D J,BONMAN J M,et al.Tagging genes for blast resistance in rice via linkage to RFLP markers[J].Theor Appl Genet,1991,81:471-476.
[8] HITTALMANI S,PARCO A,MEW T V,et al.Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice[J].Theoretical and Applied Genetics,2000,100(7):1121-1128.
[9] 陈红旗,陈宗祥,倪深,等.利用分子标记技术聚合3个稻瘟病基因改良金23B的稻瘟病抗性[J].中国水稻科学,2008,22(1):23-27.
[10] 柳武革,王丰,金素娟,等.利用分子标记辅助选择聚合Pi-1和Pi-2基因改良两系不育系稻瘟病抗性[J].作物学报,2008, 34(7):1128-1136.
[11] 柳武革,王丰,刘振荣,等.利用分子标记技术聚合Pi-1和Pi-2基因改良三系不育系荣丰A的稻瘟病抗性[J].分子植物育种2012,10(5):575-582.
[12] 曹志,曾盖,郝明,等.利用MAS技术改良水稻两用核不育系C815S的稻瘟病抗性[J].分子植物育种,2015,13(6):1193-1200.
[13] 聂元元,李霞,毛凌华,等.分子标记辅助选择改良三系杂交稻恢复系R225稻瘟病抗性[J].中国稻米,2016,22(3):60-63.
[14] 楼巧君,陈亮,罗利军.三种水稻基因组DNA快速提取方法的比较[J].分子植物育种,2005,3(5):749-752.
[15] 倪大虎,易成新,李莉,等.分子标记辅助培育水稻抗白叶枯病和稻瘟病三基因聚合系[J].作物学报,2008,34(1):100-105.
[16] 陈学伟,李仕贵,马玉清,等.水稻抗稻瘟病基因Pi-d(t)1、Pi-b、Pi-ta2的聚合及分子标记选择[J].生物工程学报,2004,20(5):708-714.
[17] 马静,陈起萱,凌文华.红、黑米的保健功效研究[J].食品科学,2000,21(12):139-140.
[18] 阎勇,莫海玲,于松保,等.优质红香稻桂红一号的选育与应用[J].作物杂志,2012(2):149-150.
[19] 唐梅,莫海玲,于松保,等.优质红色籼稻品种桂红一号特征特性及机插高产栽培技术[J].中国稻米,2012,18(12):77-78.
[20] 唐莉越,李前琦,向花香,等.红米稻柳红占211在广西山区三江县种植表现与高产栽培措施[J].农业科技通讯,2014(3):168-169.
[21] 向花香,覃凯旋,覃瑞德,等.特种红米稻柳红占214在桂中北稻区的种植表现[J].农业科技通讯,2014(8):198-199.