Optimization of extraction technique of polyphenols from Hemsleya chinensis by response surface methodology

doi:10.14088/j.cnki.issn0439-8114.2020.17.026

Abstract

Abstract: Taking Hemsleya chinensis as the experimental material, the Box-Behnken central composite design and response surface methodology were used to optimize the extraction conditions of polyphenols from Hemsleya chinensis. The extraction rate of polyphenols was used as index. Based on the results single factor experiments, a mathematical model of 4 factors and 3 levels center combinations was established with the Design-Expert 8.0.6 software on the extraction temperature, ethanol concentration, liquid to material ratio, extraction time. The results showed that the fitting degree of polyphenol extraction rate model was good and significant. The best process parameters were extraction time 59 min, ethanol concentration （V/V） 50 %, extraction temperature 74 ℃, and liquid to material ratio 16∶1（mL/g）. Under these conditions, the extraction rate of polyphenols from H. chinensis was （1.479 6±0.004 1） mg/g.

Key words: Hemsleya chinensis, polyphenols, extraction, response surface methodology

CLC Number:

R284

ZHOU Zi-shun, DUAN Ke-zhao, QUAN Rui-lan, ZHANG Guang-hui, YANG Sheng-chao, ZHAO Yan. Optimization of extraction technique of polyphenols from Hemsleya chinensis by response surface methodology[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(17): 112-118.

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