HUBEI AGRICULTURAL SCIENCES ›› 2021, Vol. 60 ›› Issue (2): 100-103.doi: 10.14088/j.cnki.issn0439-8114.2021.02.020

• Horticulture & Local Products • Previous Articles     Next Articles

Effects of different concentrations of 6-BA on the secondary culture of variant Yunyan 97

ZHANG Li-qiong, CHEN Di, YANG Song-jie, NIE Xiang-tao   

  1. School of Modern Agriculture and Biotechnology, Ankang University, Ankang 725000, Shaanxi, China
  • Received:2020-04-14 Online:2021-01-25 Published:2021-02-07

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Abstract: Two giant variants of Yunyan 97 were found in Zhenping county, Ankang city, Shaanxi province,compared with the common tobacco, these giant variants have larger growth advantage. It is a good germplasm resource to be preserved by tissue culture and rapid propagation. The effects of 6-BA concentration on the secondary culture of Yunyan 97 were studied by using tissue culture seedlings induced by axillary buds of Yunyan 97 variant as raw materials. The results showed that the suitable differentiation medium for Yunyan 97 variants was MS+6-BA 2.0 mg/L, and the third generation medium MS+6-BA 1.0 mg/L+NAA 0.2 mg/L, under these conditions, the differentiation rate of variant was 88.9%.

Key words: Yunyan 97, variant, rapid propagation, blasting rate, secondary culture

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