Construction of Cymbidium sinense AP3 gene overexpression vector and genetic transformation of Dendrobium officinale

doi:10.14088/j.cnki.issn0439-8114.2023.07.027

Abstract

Abstract: In order to explore the function of the Cymbidium sinense AP3 gene in the orchid lip, based on the AP3 gene sequence obtained from the Cymbidium sinense transcriptome in the earlier stage, the overexpression vector of the Cymbidium sinense AP3 gene through PCR technology was constructed, and the protocorm of Dendrobium officinale was transformed using Agrobacterium EHA105 to establish a genetic transformation system. After PCR verification, 9 out of 20 resistant seedlings were found to be positive, with a positive seedling rate of 45%. The Cymbidium sinense AP3 gene was successfully introduced into Dendrobium officinale.

Key words: Cymbidium sinense, AP3 gene, overexpression vector, Dendrobium officinale, genetic transformation

CLC Number:

S682.31

HE Ling, SUN Bo, WANG Yi-qin, ZANG Rui, CHEN Yu, HE Feng-mei, ZHU Yong-ping. Construction of Cymbidium sinense AP3 gene overexpression vector and genetic transformation of Dendrobium officinale[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(7): 157-162.

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