Construction and expression of genetic transformation system of Hubei potato No.3

doi:10.14088/j.cnki.issn0439-8114.2022.09.032

Abstract

Abstract: Using Hubei potato（Solanum tuberosum L.） No. 3 as the experimental material, PCR amplification was performed on the plasmid pBinAr containing selenocysteine methyltransferase gene （AbSMT） from Astragalus membranaceous. Then, the recombinant transmitter pBI121-AbSMT was constructed by enzyme digestion and ligation. The combination of Agrobacterium solution concentration gradient, infection time, and co-culture time was set for genetic transformation, and gene expression was verified by PCR identification and GUS histochemical staining. The results showed that the selected antibiotic was 50.00 mg/L Kan. Agrobacterium solution concentration was 0.50 （OD_{600 nm}）; The infection time was 8 min. Co-culture time of 3 d could realize the efficient genetic transformation of Hubei potato No. 3. At the same time, the AbSMT gene transformed Hubei potato No. 3 was successfully constructed.

Key words: A. bisulcatus selenocysteine methyltransferase, potato（Solanum tuberosum L.）, genetic transformation, expression analysis

CLC Number:

S532

GAO Zhi-peng, DU Yu-xiao, FENG Xia, YU Tu-yuan, LI Ya-nan, CHEN Da-qing. Construction and expression of genetic transformation system of Hubei potato No.3[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(9): 162-167.

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