Creation of radish cytoplasmic male sterility （Ogura CMS） Chinese cabbage breeding material with heat tolerance, clubroot resistance

doi:10.14088/j.cnki.issn0439-8114.2024.12.015

Abstract

Abstract: In order to obtain Ogura cytoplasmic male sterile Chinese cabbage materials with heat resistance and clubroot resistance, F₁ was obtained by crossing heat resistant Chinese cabbage as female parent and clubroot resistant baby cabbage as male parent. After identification, CMSF₁ was obtained by crossing with Ogura cytoplasmic male sterile baby cabbage with clubroot resistance. Then, F₂ obtained by self-crossing F₁ was selected as male parent, and CMSF₁ was selected as female parent for backcrossing to obtain BC₁. The results of phenotypic identification of clubroot resistance showed that F₁, CMSF₁ and BC₁ were resistant to clubroot. The results of molecular marker identification showed that F₁ plants carried clubroot resistance genes, and most of CMSF₁and BC₁ plants carried clubroot resistance genes. The results of heat tolerance identification showed that the increase of SOD, POD and CAT activity of F₁, CMSF₁ and BC₁ under high temperature stress was greater than that of Mini Yellow 1. The floral organ observation of CMSF₁ and BC₁ showed that the stamen abortion was without pollen, which was Ogura cytoplasmic male sterile strain.

Key words: Chinese cabbage, clubroot resistance, heat tolerance, Ogura CMS

CLC Number:

S634.1

ZHAO Ya-lan, HU Jing-feng, YANG Hong-li, DONG Xiang-shu, HE Jiang-ming. Creation of radish cytoplasmic male sterility （Ogura CMS） Chinese cabbage breeding material with heat tolerance, clubroot resistance[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(12): 81-86.

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