GC-MS analysis of differences in volatile oil components of three traditional Chinese medicines in Alpinia

doi:10.14088/j.cnki.issn0439-8114.2025.01.023

Abstract

Abstract: Using ultrasound assisted solvent method and reflux method, the chemical components of volatile oils from three traditional Chinese medicines of Alpinia （Gaoliangjiang, Dagaoliangjiang, and Hongdoukou） were analyzed by gas chromatography-mass spectrometry （GC-MS）. The relative content of each component was calculated by peak area normalization, and principal component analysis （PCA） and cluster analysis were performed using Simca 14.1 and SPSS 26.0 software, respectively. The results showed that 134, 130, and 171 components were identified in Gaoliangjiang, Dagaoliangjiang, and Hongdoukou, respectively;there were a total of 24 components in the volatile oils of three traditional Chinese medicines in Alpinia. The three traditional Chinese medicines of Alpinia were mainly composed of terpenes, fatty hydrocarbons, aromatic hydrocarbons, oxygen-containing compounds, phenylpropanoids, etc. （S） 4- （1-Acetylally） phenyl acetate （N31）, 1H-Imidazole, 4,5-dihydro-2- （phenylmethyl） - （N56）, and 3-Heptanone, 5-hydroxy-1,7-diphenyl - （N100） were chemical markers of the volatile oils in three traditional Chinese medicines of Alpinia. The common components of Gaoliangjiang, Dagaoliangjiang, and Hongdoukou were 27, 3, and 24, respectively, accounting for 20.15%, 2.31%, and 14.04%, respectively. Through the analysis of the main components, common components, and unique components of the volatile oils from three traditional Chinese medicines of Alpinia, it was shown that the material basis of Chinese medicines with similar genetic relationships was correlated.

Key words: traditional Chinese medicine of Alpinia, volatile oil, GC-MS combined technology, components, differences

CLC Number:

R284

CHEN Zhi-lan, NIU Xin-mai, XIE Xu-ge, HUANG Shao-min, GAN Jiao-jiao, CHEN Mei-an, QIN Hua-zhen. GC-MS analysis of differences in volatile oil components of three traditional Chinese medicines in Alpinia[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(1): 133-149.

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