Callus induction and plant regeneration from different explants of Aquilaria sinensis

doi:10.14088/j.cnki.issn0439-8114.2025.12.038

Abstract

Abstract: Using the leaf,stem,bud, and root of Aquilaria sinensis(Lour.) Gilg. as experimental materials, an orthogonal experimental design was employed via DPS software. By applying different types and concentrations of plant growth regulators (6-BA, 2,4-D, TDZ, NAA, KT), the effects of growth regulators combinations and their concentrations on callus induction, differentiation, and rooting of Aquilaria sinensis were investigated. The results showed that the optimal explant for inducing Aquilaria sinensiscalluswas theleaf, with the highest induction rate of 100%. The optimal medium for leafcallus induction was WPM + 6-BA 1.5 mg/L + 2,4-D 0.5 mg/L + NAA 0.8 mg/L + KT 0.2 mg/L + TDZ 1.0 mg/L. The optimal medium for adventitious bud induction ofAquilaria sinensiswas WPM + 6-BA 0.5 mg/L + NAA 0.2 mg/L + sucrose 20 g/L + agar 6 g/L. The callus from buds and stems could differentiate into adventitious buds. The optimal medium for adventitious bud induction was WPM + 6-BA 0.5 mg/L + NAA 0.2 mg/L + sucrose 20 g/L + agar 6 g/L, resulting in a bud induction rate of 72.22% and an average of 5.68 adventitious buds per explant. The optimal medium for root induction was 1/2 MS + NAA 1.0 mg/L + sucrose 20 g/L + agar 6 g/L, achieving a rooting rate of 46.67%.

Key words: Aquilaria sinensis(Lour.) Gilg., explants, callus induction, plant regeneration, orthogonal test

CLC Number:

S567.1⁺9

YUAN Chang-ge, ZHOU Ya-qin, QIN Shou-jie, ZHANG Miao, FU Peng, TAN Xiao-ming. Callus induction and plant regeneration from different explants of Aquilaria sinensis[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(12): 228-234.

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