Identification and expression analysis of the alternative oxidase family gene in Amorphophallus konjac

doi:10.14088/j.cnki.issn0439-8114.2025.12.039

Abstract

Abstract: The alternative oxidase（AOX） family gene in Amorphophallus konjac K.Koch was analyzed at the whole-genome level. The results showed that there were four alternative oxidase family gene members in Amorphophallus konjac, named AkAOX1 to AkAOX4. Their protein length ranged from 330 to 361 aa, molecular weights from 37 087.52 to 41 243.83 u, and isoelectric points from 5.97 to 7.27. They were primarily located in the cytoplasm and chloroplast. The Amorphophallus konjac alternative oxidase family gene members were divided into two subfamilies, namely the AOX1 subfamily (AkAOX1, AkAOX2, AkAOX3) and the AOX2 subfamily (AkAOX4). Gene collinearity analysis indicated no potential duplicated gene pairs or tandem duplication events in Amorphophallus konjac. Ka/Ks analysis further showed that these homologous gene pairs were primarily under purifying selection during evolution. The promoter regions of the Amorphophallus konjac alternative oxidase family gene contained three types of cis-acting elements, associated with plant hormone response, growth and development response, and environmental (stress) response. The qRT-PCR results showed that the expression level of the AkAOX1 gene was significantly up-regulated under soft rot pathogen infection, drought stress, and salt stress, while the expression level of the AkAOX4 gene was significantly down-regulated under soft rot pathogen infection. It was speculated that the Amorphophallus konjac alternative oxidase family gene played important roles in its growth and development, hormone signal transduction, and responses to biotic and abiotic stresses.

Key words: Amorphophallus konjac K.Koch, alternative oxidase（AOX）, family gene, identification, expression analysis

CLC Number:

Q944.6

GUAN Wan-ting, HE Fei, LUO Chang-xin, YI Qian, ZHAO Xiao-jie, PU Xing-yan, SUN Ke-xin, ZHU Feng-yan, CHU Hong-long. Identification and expression analysis of the alternative oxidase family gene in Amorphophallus konjac[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(12): 235-243.

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