Identification and expression analysis of members in the EIL gene family of wild barley

doi:10.14088/j.cnki.issn0439-8114.2025.08.034

Abstract

Abstract: Members of the EIL gene family in wild barley (Hordeum spontaneum) were identified based on whole-genome analysis technology; the composition and distribution characteristics of cis-acting elements in their promoter regions were analyzed; and their expression patterns under stress conditions were investigated to mine potential stress-resistant regulatory genes. The results showed that the EIL gene family in wild barley contained seven members, namely HvEIL1, HvEIL2, HvEIL3, HvEIL4, HvEIL5, HvEIL6, and HvEIL7. The seven members had an average protein length of 489 aa, a mean molecular weight of 53 790.52 u, isoelectric points ranging from 4.89 to 6.27, and instability indices between 42.66 and 60.39; all were hydrophilic proteins and localized in the nucleus. Cis-acting elements in the promoter regions of members in the EIL gene family of wild barley totaled 14 types, mainly including light-responsive elements, plant hormone-responsive elements, and stress-responsive elements. In transcriptome data analysis of wild barley, gene expression levels varied under different treatments. HvEIL2, HvEIL3, and HvEIL7 showed no significant differences in expression levels under control treatment, drought stress treatment, or rehydration treatment after drought, indicating that these genes might not participate in drought stress response. The suppression of HvEIL4 expression was associated with enhanced drought resistance, suggesting that it might play a negative regulatory role in barley drought tolerance.

Key words: wild barley (Hordeum spontaneum), members of the EIL gene family, identification, expression analysis

CLC Number:

S512

HU Hui, MEN Rui-long, ZHAO Ming-zhuo, SUN Ming-ruo, XU Jun-ying, LIU Hai-yang, YANG Long-wei, TIAN Yu, YU Wei-pu, QIU Xian-jin. Identification and expression analysis of members in the EIL gene family of wild barley[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(8): 226-229.

References

[1] HUSSAIN S,HUSSAIN S,QADIR T,et al.Drought stress in plants: An overview on implications, tolerance mechanisms and agronomic mitigation strategies[J].Plant science today,2019, 6(4): 389-402.
[2] WU W, YAN Y.Chloroplast proteome analysis of Nicotiana tabacum overexpressing TERF1 under drought stress condition[J].Botanical studies, 2018, 59: 1-12.
[3] YASEEN R, AZIZ O, SALEEM M H, et al.Ameliorating the drought stress for wheat growth through application of ACC-deaminase containing rhizobacteria along with biogas slurry[J]. Sustainability, 2020, 12(15): 6022.
[4] GAO Y, HAN D, JIA W, et al.Molecular characterization and systematic analysis of NtAP2/ERF in tobacco and functional determination of NtRAV-4 under drought stress[J]. Plant physiology and biochemistry, 2020, 156: 420-435.
[5] NAZIR F, PETER P, GUPTA R, et al.Plant hormone ethylene: A leading edge in conferring drought stress tolerance[J]. Physiologia plantarum, 2024, 176(1): e14151.
[6] AN F Y, ZHANG X, ZHU Z Q, et al.Coordinated regulation of apical hook development by gibberellins and ethylene in etiolated Arabidopsis seedlings[J]. Cell research, 2012, 22(5): 915-927.
[7] CHEN H M, XUE L, CHINTAMANANI S, et al.Ethylene insensitive3 and ethylene insensitive3-like1 repress salicylic acid induction deficient2 expression to negatively regulate plant innate immunity in Arabidopsis[J]. Plant cell, 2009,21(8): 2527-2540.
[8] ZHU Z Q, AN F Y, FENG Y, et al.Derepression of ethylene-stabilized transcription factors (EIN3/EIL1) mediates jasmonate and ethylene signaling synergy in Arabidopsis[J]. Proceedings of the national academy of sciences, 2011, 108(30): 12539-12544.
[9] HUSAIN T, FATIMA A, SUHEL M, et al.A brief appraisal of ethylene signaling under abiotic stress in plants[J]. Plant Signal Behav, 2020, 15(9): 1782051.
[10] HIRAGA S, SASAKI K, HIBI T, et al.Involvement of two rice ethylene insensitive3-like genes in wound signaling[J]. Mol Genet Genomics, 2009, 282: 517-529.
[11] PAN R, HU H F, XIAO, Y H, et al.High-quality wild barley genome assemblies and annotation with Nanopore long reads and Hi-C sequencing data[J]. Sci data, 2023, 10(1): 535.
[12] JIN J, TIAN F, YANG D C, et al.PlantTFDB 4.0: Toward a central hub for transcription factors and regulatory interactions in plants[J]. Nucl Acid Res, 2017, 45: D1040-D1045.
[13] POTTER S C, LUCIANI A, EDDY S R, et al.HMMER web server: 2018 update[J]. Nucl Acid Res, 2018, 46(1): 200-204.
[14] CHEN C J, CHEN H, ZHANG Y, et al.TBtools: An integrative toolkit developed for interactive analyses of big biological data[J].Mol plant, 2020, 13(8): 1194-1202.
[15] ZHU J K.Abiotic stress signaling and responses in plants[J]. Cell, 2016, 167(2): 313-324.
[16] WOODWARD A W, BARTEL B.Auxin: Regulation, action, and interaction[J]. Ann Bot, 2005, 95(5): 707-735.