Physiological and biochemical studies on germination dynamics of Taxillus chinensis seeds

doi:10.14088/j.cnki.issn0439-8114.2025.05.013

Abstract

Abstract: Using Taxillus chinensis seeds with pectin as the control group and de-pectinated seeds as the treated group, samples were collected at 0, 5, 10, 15, 20, and 25 days of cultivation. Then relevant physiological and biochemical indicators were determined and comparatively analyzed. The results showed that the polysaccharide content in the control exhibited an initial increase and then followed by a decrease, while the de-pectinated treatment showed a continuous declining trend. The soluble sugar content in both groups displayed a decrease-increase-decrease pattern, with significantly lower levels observed in the de-pectinated treatment compared to the control throughout the germination process. The MDA content in the control group showed an upward trend, while in the de-pectinated group, a downward-upward-downward trend was exhibited and it was mostly lower than that in the control during germination. The content of H₂O₂ and O₂- showed similar trends in both groups, but the content in the de-pectinated group was mostly higher than that in the control during germination. The activities of SOD, POD and PAL exhibited similar trends in both groups. The SOD activity in the de-pectinated group was consistently higher than that in the control, while POD and PAL activities, although lower than those in the control, showed a continuous upward trend. The endogenous hormone content (IAA, ABA, GA₃, ZR) exhibited fluctuating changes in both groups, with the control group showing a general downward trend, while the de-pectinated group showed a significant increase and a general upward trend. It was preliminarily confirmed that pectin removal could promote the rapid mobilization of stored substances, enzymes and endogenous hormones in the seeds of Taxillus chinensis, promoting the smooth germination of seeds.

Key words: Taxillus chinensis, seed, pectin, germination process, physiological and biochemical indicators

CLC Number:

FENG Chang-cun, LIANG Lu, LIANG Wen-jing, GUI Ling-jian, SONG Li-sha, PAN Li-mei, FU Jin-e, WAN Ling-yun, WEI Shu-gen. Physiological and biochemical studies on germination dynamics of Taxillus chinensis seeds[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(5): 83-89.

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