Analysis of endophytic fungal diversity of Centella asiatica of Guangxi

doi:10.14088/j.cnki.issn0439-8114.2025.05.014

Abstract

Abstract: In order to investigate the distribution of endophytic fungi in the roots, stems and leaves of Centella asiatica (L.) Urban, the composition of endophytic fungi groups of Centella asiatica in Guangxi was analyzed. Healthy Centella asiatica specimens were collected from six distinct regions in Guangxi, including Liuzhou, Qinzhou, Beihai, Guilin, Hezhou, and Baise cities, followed by endophytic fungal isolation from their root, stem, and leaf tissues. The endophytic fungi were identified by combining morphological and molecular biological methods, followed by diversity analysis. The results showed that a total of 114 endophytic fungal strains were isolated, belonging to 1 phylum, 3 classes, 9 orders, 12 families, and 17 genera, with the taxonomic status of 1 genus remaining undetermined. The genera Colletotrichum and Diaporthe were identified as the dominant endophytic fungi in Centella asiatica. The endophytic fungi diversity of Centella asiatica in different regions was significantly different. The endophytic fungi in Guilin City and Liuzhou City were the most abundant, and the endophytic fungi in Beihai City and Qinzhou City were the most similar, followed by Guilin City and Liuzhou City. Through comparative analysis, it was found that the endophytic fungi in roots had the highest abundance, and the similarity coefficient of endophytic fungi in stems and leaves was the highest. The findings indicated that Centella asiatica in Guangxi exhibited high diversity and relatively uniform distribution of endophytic fungi, with both growth environment and plant tissue type significantly influencing their community composition and diversity.

Key words: Centella asiatica, endophytic fungi, isolation and identification, diversity, Guangxi

CLC Number:

LIANG Lu, LIANG Wen-jing, SONG Li-sha, WAN Ling-yun, TAN Gui-yu, ZHANG Zhan-jiang, PAN Li-mei, ZHANG Kun, TU Dong-ping, WEI Shu-gen. Analysis of endophytic fungal diversity of Centella asiatica of Guangxi[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(5): 90-101.

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