Abstract: In order to breed new varieties of flue-cured tobacco resistant to herbicide, the NtALS gene of flue-cured tobacco K326 was edited by the single base, the 194th proline triad code CCA of tobacco NtALS gene was replaced with leucine encoding CTA and TTA. Sequencing results showed that the editing rate was up to 20.69%, the identification results of the herbicide spraying resistance of positive edited plants showed that the resistance was obvious, and a new flue-cured tobacco material with herbicide resistance was developed.

Key words: acetolactate synthase(ALS), tobacco K326, herbicide, genome editing