Research on molecular mechanisms of Oryza sativa L. response to waterlogging stress during tillering stage using integrated transcriptome-metabolome analysis

doi:10.14088/j.cnki.issn0439-8114.2025.06.036

Abstract

Abstract: Using Oryza sativa L. cultivars Ezhong 6 (indica) and Changligeng 384 (japonica) as materials, plants at the tillering stage were subjected to 2/3 plant height water depth treatment (lasting 7 days). Transcriptome and metabolome analyses were performed to investigate post-stress gene expression and metabolite changes.Transcriptome analysis identified 3 080 and 1 624 differentially expressed genes (DEGs) in Ezhong 6 and Changligeng 384, respectively, which were significantly enriched in carbohydrate biosynthetic process, reactive oxygen species metabolic process, plant-pathogen interaction, and plant hormone signal transduction pathways.Metabolome analysis revealed significant enrichment of differentially accumulated metabolites (DAMs) such as benzene and substituted derivatives, organic acids, and lactones in Ezhong 6 and Changligeng 384, primarily involved in diterpenoid biosynthesis and flavonoid biosynthesis pathways.Integrated transcriptome-metabolome analysis identified seven co-enriched pathways in both cultivars: Metabolic pathways, biosynthesis of secondary metabolites, biosynthesis of various plant secondary metabolites, diterpenoid biosynthesis, linoleic acid metabolism, phenylpropanoid biosynthesis, and carotenoid biosynthesis.Oryza sativa L. responded to waterlogging stress by activating antioxidant systems, regulating hormone signaling, and accumulating defensive metabolites.

Key words: transcriptome, metabolome, waterlogging stress, rice (Oryza sativa L.), tillering stage, molecular mechanisms

CLC Number:

S332.4

LUO Xiao-yun, LI Pei-de, ZHENG Xing-fei, YIN De-suo, WANG Hong-bo, HU Jian-lin, HU Peng, LIU Dan, WEN Yi, CHEN Dong-pan, LEI Tian-jie, XU De-ze. Research on molecular mechanisms of Oryza sativa L. response to waterlogging stress during tillering stage using integrated transcriptome-metabolome analysis[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(6): 220-231.

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