The Content of Four Kinds of Tocopherol in Rice Bran Oil was Determined by Reversed-phase Liquid Chromatography

doi:10.14088/j.cnki.issn0439-8114.2018.11.021

Abstract

Abstract: The method of simultaneous determination of 4 kinds of tocopherol in vegetable oil was established by reversed-phase liquid chromatography.After sample ultrasonic extraction with methanol,centrifugal supernatant liquid nitrogen blow to dry,the capacity after the methanol organic phase filter membrane,PEF column（150×3.0 mm,2.6microns） separation,methanol and water （88∶12） elution flow equal degree,velocity 0.4 mL/min;Column temperature 30 ℃;The fluorescence detection （a） of the sample volume was 5 μL,the external standard method was quantitatively determined,and the two types of paroxygenol isomers were isolated at the same time in 20 min. The mass concentration of 4 kinds of tocopherol was in a good linear relationship with the peak area within the range of 0.5~50.0 μg/mL,and the linear correlation coefficient was greater than 0.999. Adding in the three levels with respect to the average recovery was 84.47%~116.50%,relative standard deviation（RSD） for 6.0% or less（n=6）,alpha tocopherol,beta tocopherol,gamma tocopherol and delta-tocopherol,method detection limit （LOD） were respectively 0.10,0.05,0.05,0.04 mg/kg. The method is accurate,sensitive and reliable,and can be applied to the analysis of actual samples.

Key words: inverse phase high performance liquid chromatography, fluorescence detection, tocopherol, rice bran oil

CLC Number:

O657.7⁺2

LI Qing, ZHOU Xiao-qing, CHEN Sha, YU Jun-lei. The Content of Four Kinds of Tocopherol in Rice Bran Oil was Determined by Reversed-phase Liquid Chromatography[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(11): 87-90.

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