HUBEI AGRICULTURAL SCIENCES ›› 2020, Vol. 59 ›› Issue (17): 175-177.doi: 10.14088/j.cnki.issn0439-8114.2020.17.040

• Biological Engineering • Previous Articles     Next Articles

Rapid identification of differential protein related to rice quality by iTRAQ

ZHA Wen-jun1, YOU Ai-qing1,2   

  1. 1. Food Crops Institute,Hubei Academy of Agricultural Sciences/Hubei Key Laboratory of Food Crop Germplasms and Genetic Improvement,Wuhan 430064;
    2. Hubei Collaborative Innovation Center for Grain Industry,Yangtze University,Jingzhou 434025,Hubei,China
  • Received:2020-02-18 Published:2020-10-23

PDF

211

Abstract: Using Ezhong No. 5 and Guichao No. 2 as materials, the iTRAQ technology was used to quickly identify rice quality differential proteins, so that candidate genes related to rice quality can be rapidly and effectively screened based on the differential proteins. The results showed that Ezhong No. 5 and Guichao No. 2 identified a total of 61 significantly different proteins in rice, including 21 up-regulated proteins and 40 down-regulated proteins. This technology can be widely used in the research and development of rice quality traits and combined with transgenic technology to cultivate high-quality rice varieties.

Key words: rice（Oryza sativa L.）, iTRAQ, differential protein, identify

CLC Number: