Analysis of endogenous harmful components in ginkgo seed kernel and fried ginkgo seed kernel

doi:10.14088/j.cnki.issn0439-8114.2023.05.027

Abstract

Abstract: Ginkgo seed kernel and fried ginkgo seed kernel were used as experimental materials for the determination of total ginkgolic acid using HPLC. The chromatographic column was YMC-Triart C18 （4.6 mm×150 mm,5 μm）. The mobile phase was acetonitrile solution with 0.1% trifluoroacetic acid -0.1% trifluoroacetic acid aqueous solution, with a detection wavelength of 310 nm and a flow rate of 1.0 mL/min; 4′-O-methyl pyridoxine （MPN） and 4′-O-methoxy pyridoxin-5′- glucoside （MPNG） were determined by HPLC on YMC Triart C18 （4.6 mm×150 mm,5 μm）. The mobile phase was methanol deionized water, with a detection wavelength of 328 nm and a flow rate of 1.0 mL/min. Headspace gas chromatography （HS-GC） was used to perform a limit check on hydrocyanic acid. The results showed that the average recovery rate of total ginkgolic acid was 101.17%, RSD was 4.57% （n=6）, the quantification limit was 12.18 ng, and the detection limit was 4.87 ng; the average recovery rate of MPN was 103.14%, RSD was 4.80% （n=6）, the quantification limit was 5.94 ng, and the detection limit was 2.38 ng; the average recovery rate of MPNG was 103.54%, RSD was 3.05% （n=6）, the quantification limit was 11.20 ng, and the detection limit was 4.48 ng; the quantitative limit of hydrocyanic acid was 10 ng, and the detection limit was 3.5 ng. The established method had strong specificity, good repeatability, and high sensitivity, and could be used for the detection of endogenous harmful components in ginkgo seed kernel and fried ginkgo seed kernel.

Key words: ginkgo seed kernel, fried ginkgo seed kernel, total ginkgolic acid, MPN, MPNG, cyanoside, Ginkgo biloba L., content determination

CLC Number:

R284

ZHANG Yan-miao, DING Ming-he, ZHANG Ying, TAN Zhong-chuan, ZHOU Tian-tian, GAN Guo-ping. Analysis of endogenous harmful components in ginkgo seed kernel and fried ginkgo seed kernel[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(5): 153-159.

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