Isolation of endophytic bacteria from Morinda citrifolia and antioxidant activity of its metabolites

doi:10.14088/j.cnki.issn0439-8114.2025.01.011

Abstract

Abstract: Nine endophytic bacteria were isolated from fresh Morinda citrifolia, which were cultured in the liquid medium respectively. The supernatant obtained after centrifuging the culture broth was the extracellular metabolite, and the intracellular metabolite （intracellular content） was obtained by ultrasonic cell disruption of the centrifuged bacteria. The scavenging effects on DPPH free radicals, hydroxyl free radicals, superoxide anions, hydrogen peroxide and the total reducing power of the extracellular metabolites and intracellular contents were tested. The results showed that the antioxidant activities of the extracellular metabolites and intracellular contents of the nine endophytic bacteria strains from Morinda citrifolia were different. Among them, the scavenging rate of the extracellular metabolites of 3# endophyte on DPPH free radicals reached 95.49%, and that of the intracellular content of 4# endophyte on DPPH free radicals reached 92.47%, both exceeding that of 90 μmol/L ascorbic acid of the same volume. The scavenging effect of the extracellular metabolites of 2# endophyte on hydroxyl free radicals was far beyond that of ascorbic acid, reaching 98.13%. The scavenging rate of hydroxyl free radicals of the intracellular content of 3# endophyte was 78.00%, which was twice that of ascorbic acid. The scavenging rate of the extracellular metabolite of 3# endophyte on superoxide anions reached 100%. The total reducing power of the intracellular content of 4# endophyte was the highest.

Key words: Morinda citrifolia, endogenous bacteria, metabolite, antioxidant activity

CLC Number:

Q936

ZHANG San-yan, CHEN Xue-ru, JIN Wei-hua. Isolation of endophytic bacteria from Morinda citrifolia and antioxidant activity of its metabolites[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(1): 59-64.

References

[1] THOO Y Y,HO S K,ABAS F,et al.Optimal binary solvent extraction system for phenolic antioxidants from mengkudu (Morinda citrifolia) Fruit[J].Molecules,2013,18(6):7004-7022.
[2] WANG M Y,SU C.Cancer preventive effect of Morinda citrifolia(Noni)[J].Annals of the New York academy of sciences,2010,952:161-168.
[3] 王丽. 诺丽果的营养与保健功能[J].中国妇幼健康研究,2017,28(S4):139-140.
[4] 范海能,苏定志,王谦,等.诺丽果发酵液抑制肝癌细胞Bel7402增殖及促凋亡作用观察[J].中医药学报,2018,46(6):48-51.
[5] TORRES M A O,DE FÁTIMA BRAGA MAGALHÃES I,MONDÊGO-OLIVEIRA R,et al. One plant, many uses: A review of the pharmacological applications of Morinda citrifolia[J].Phytother Res,2017,31(7):971-979.
[6] LIM S L,GOH Y M,NOORDIN M M,et al.Morinda citrifolia edible leaf extract enhanced immune response against lung cancer[J].Food & function,2016,7(2):741-751.
[7] 刘霭莎,洪启恩,冯兴江,等.诺丽果、种子和叶提取物抑菌活性及复合植物抑菌液配方筛选[J].食品工业科技,2017,38(14):171-173,187.
[8] 林金莺,刘鹏,方韵婷,等.诺丽酵素长效抗自由基的探究[J].食品工业,2017,38(4):222-225.
[9] 田亮,孙碧琪,毛翔飞,等.不同品种诺丽果发酵前后品质、挥发性风味成分和抗氧化活性比较[J].中国酿造,2023,42(9):138-143.
[10] 孙碧琪,史涵博,田亮,等.4种海南诺丽果内生细菌多样性及其关键生物活性研究[J].食品科学技术学报,2023,41(3):85-97.
[11] 徐重新,仲剑锋,高美静,等.植物内生菌在食用农产品质量安全与营养品质调控中的研究进展[J].生物技术通报,2022, 38(5):215-227.
[12] SAHU P K,TILGAM J,MISHRA S,et al.Surface sterilization for isolation of endophytes: Ensuring what (not) to grow[J].Journal of basic microbiology,2022,62(6):647-668.
[13] 冯沼润,何先元,欧阳凯,等.响应面法优化天葵子总生物碱提取工艺及其体外抗氧化活性研究[J].中药新药与临床药理,2018,29(2):199-204.
[14] KHAN B M,QIU H M,XU S Y,et al.Physicochemical characterization and antioxidant activity of sulphated polysaccharides derived from Porphyra haitanensis[J].International journal of biological macromolecules, 2020,145:1155-1161.
[15] RAJAURIA G,JAISWAL A K,ABU-GANNAM N,et al.Antimicrobialantioxidant and free radical-scavenging capacity of brown seaweed himanthalia elongata from western coast of ireland[J].Journal of food biochemistry,2013,37(3):322-335.
[16] 李奕星,袁德保,郑晓燕,等.诺丽果汁的抗氧化性研究[J].热带作物学报,2013,34(8):1531-1534.
[17] 赵宏亮,杨晓红,张伟敏.诺丽果渣成分分析及抗氧化性研究[J].食品安全质量检测学报,2019,10(2):358-364.
[18] 曾维才,石碧.天然产物抗氧化活性的常见评价方法[J].化工进展,2013,32(6):1205-1213,1247.
[19] 董琛琳,刘娜,王园,等.发酵米糠水提物的抗氧化活性研究[J].饲料工业,2021,42(24):8-13.
[20] 郝玉洁,毛丙永,崔树茂,等.诺丽果控温发酵过程中理化性质和活性物质的变化规律[J].食品与发酵工业,2018,44(9):105-109.