Effects of acetochlor and butachlor on prostaglandin metabolites in zebrafish

doi:10.14088/j.cnki.issn0439-8114.2025.06.017

Abstract

Abstract: Adult female and male zebrafish(Danio rerio) were exposed to 100 μg/L of acetochlor(AC) and butachlor (BC) for 96 h, and the effects of acetochlor or butachlor on the prostaglandin (PGs) metabolites of zebrafish were investigated using metabolomics techniques. The results showed that an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) detection method was established, and a total of 1 683 metabolites were detected from the 24 samples of the six treatments[AC treated female(ACF) and male fish(ACM),BC treated female（BCF）and male fish(BCM),control female(CKF) and male fish(CKM)]. Volcano plot analysis of all metabolites revealed that except for BCM and CKM treatments,the number of significantly up-regulated metabolites was notably higher than that of down-regulated ones. Prostaglandin metabolite (PGs) analysis showed that under ACF treatment, 16 PGs metabolites were significantly up-regulated, and iPGs were significantly down-regulated; under ACM treatment, 9 PGs metabolites were significantly up-regulated. After BCF treatment, 18 PGs metabolites were significantly up-regulated; after BCM treatment, 2 PGs metabolites were significantly up-regulated.

Key words: zebrafish(Danio rerio), prostaglandins, metabolites, acetochlor, butachlor

CLC Number:

ZHOU Xin-zong, LI Shao-bin, TIAN Xiao-li, CHANG Ju-hua. Effects of acetochlor and butachlor on prostaglandin metabolites in zebrafish[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(6): 103-109.

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